TY - JOUR
T1 - Early Interrogation and Recognition of DNA Sequence by Indirect Readout
AU - Little, Elizabeth J.
AU - Babic, Andrea C.
AU - Horton, Nancy C.
N1 - Funding Information:
We thank J. Martinez for graphic support. This work was supported by NIH grant 5R01GM066805 (to N.C.H.). Portions of this work were carried out at the Advanced Light Source. The Advanced Light Source is supported by the director (Office of Science, Office of Basic Energy Sciences) of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
PY - 2008/12/12
Y1 - 2008/12/12
N2 - Control of replication, transcription, recombination and repair requires proteins capable of finding particular DNA sequences in a background of a large excess of nonspecific sequences. Such recognition can involve direct readout, with direct contacts to the bases of DNA, or in some cases through the less well-characterized indirect readout mechanisms. In order to measure the relative contributions of direct and indirect readout by a sequence specific endonuclease, HincII, a mutant enzyme deficient in a direct contact, was characterized, and surprisingly showed no loss of sequence specificity. The three dimensional crystal structure shows the loss of most of the direct readout contacts to the DNA, possibly capturing an early stage in target site recognition using predominately indirect readout to prescreen sites before full sequence interrogation.
AB - Control of replication, transcription, recombination and repair requires proteins capable of finding particular DNA sequences in a background of a large excess of nonspecific sequences. Such recognition can involve direct readout, with direct contacts to the bases of DNA, or in some cases through the less well-characterized indirect readout mechanisms. In order to measure the relative contributions of direct and indirect readout by a sequence specific endonuclease, HincII, a mutant enzyme deficient in a direct contact, was characterized, and surprisingly showed no loss of sequence specificity. The three dimensional crystal structure shows the loss of most of the direct readout contacts to the DNA, possibly capturing an early stage in target site recognition using predominately indirect readout to prescreen sites before full sequence interrogation.
KW - DNA
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U2 - 10.1016/j.str.2008.09.009
DO - 10.1016/j.str.2008.09.009
M3 - Article
C2 - 19081059
AN - SCOPUS:57049101786
SN - 0969-2126
VL - 16
SP - 1828
EP - 1837
JO - Structure
JF - Structure
IS - 12
ER -