Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN-γ/TNF-α in HT-29 cells

Norbert W. Lutz; Margaret E. Tome; Patrick J. Cozzone

doi:10.1016/S0014-5793(03)00489-7

Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN-γ/TNF-α in HT-29 cells

Norbert W. Lutz, Margaret E. Tome, Patrick J. Cozzone

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by ¹H and ³¹P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5'-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.

Original language	English (US)
Pages (from-to)	123-128
Number of pages	6
Journal	FEBS Letters
Volume	544
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00489-7
State	Published - Jun 5 2003
Externally published	Yes

Keywords

Apoptosis
Cytokine treatment
HT-29 cells
Metabolism
Nuclear magnetic resonance spectroscopy

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)00489-7

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title = "Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN-γ/TNF-α in HT-29 cells",

abstract = "The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5'-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.",

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AU - Lutz, Norbert W.

AU - Tome, Margaret E.

AU - Cozzone, Patrick J.

PY - 2003/6/5

Y1 - 2003/6/5

N2 - The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5'-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.

AB - The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5'-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.

KW - Apoptosis

KW - Cytokine treatment

KW - HT-29 cells

KW - Metabolism

KW - Nuclear magnetic resonance spectroscopy

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ER -

Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN-γ/TNF-α in HT-29 cells

Abstract

Keywords

ASJC Scopus subject areas

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