Dual roles of amniotic fluid nitric oxide and prostaglandin E2 in preterm labor with intra-amniotic infection

Chaur Dong Hsu, Erika Meaddough, Kristen Aversa, Shih Fen Hong, In Sik Lee, Ray O. Bahodo-Singh, Li Cheng Lu, Joshua A. Copel

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We hypothesized that induction of nitric oxide synthase and cyclo- oxygenase-2 by bacterial products in intra-amniotic infection could increase the production of proinflammatory nitric oxide and prostaglandin E2 (PGE2) and cause preterm labor. Thus, we sought to determine amniotic fluid levels of nitric oxide metabolites (NOx) and PGE2 in preterm labor patients with and without intra-amniotic infection. Amniotic fluid from 13 preterm labor patients with intra-amniotic infection and 24 without intra-amniotic infection were studied. Intra-amniotic infection was defined as the presence of a positive amniotic fluid culture. Amniotic fluid was tested for NOx, PGE2, glucose, leukocyte counts, Gram stains, creatinine, pH, and specific gravity. NOx was determined using Griess reagent after reduction of nitrate to nitrite with aspergillus nitrate reductase. PGE2 was measured by an enzyme-linked immunoassay. Both amniotic fluid NOx and PGE2 were normalized by amniotic fluid creatinine. We found that amniotic fluid concentrations of NOx and PGE2 were significantly higher in preterm labor patients with intra- amniotic infection compared to those without intra-amniotic infection (NOx: median 1.8 μmol/mg creatinine, range 0.7 to 6.8 vs. 1.3 μmol/mg creatinine, range 0.9 to 2.1, p = 0.03; PGE2: median 33.5 ng/mg creatinine, range 0.0 to 1048.6 vs. 0.0 ng/mg creatinine, range 0.0 to 33.6, p = 0.004). In addition, amniotic fluid NOx and PGE2 were positively correlated (r = 0.343, p = 0.0398). We conclude that there may be an interaction between the nitric oxide and prostaglandin pathways in intraamniotic infection. Increased production of amniotic fluid pro-inflammatory nitric oxide and PGE2 may play an important role in the pathogenesis of preterm labor in patients with intra-amniotic infection.

Original languageEnglish (US)
Pages (from-to)683-687
Number of pages5
JournalAmerican Journal of Perinatology
Volume15
Issue number12
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Amniotic fluid
  • Intra-amniotic infection
  • Nitric oxide
  • Preterm labor
  • Prostaglandin E

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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