TY - JOUR
T1 - Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy
AU - Barton, Jennifer Kehlet
AU - Guzman, Francisco
AU - Tumlinson, Alexandre
N1 - Funding Information:
The authors appreciate the assistance of Ms. Faith Rice and Dr. Stuart Williams in preparation and interpretation of histology, and University Medical Center pathology for the samples. This work was supported in part by grants from the National Science Foundation (9978820) and the National Institutes of Health (CA83148). Tissue was obtained from the National Disease Research Interchange.
PY - 2004/5
Y1 - 2004/5
N2 - We develop a dual-modality device that combines the anatomical imaging capabilities of optical coherence tomography (OCT) with the functional capabilities of laser-induced fluorescence (LIF) spectroscopy. OCT provides cross-sectional images of tissue structure to a depth of up to 2 mm with approximately 10-μm resolution. LIF spectroscopy provides histochemical information in the form of emission spectra from a given tissue location. The OCT subsystem utilizes a superluminescent diode with a center wavelength of 1300 nm, whereas a helium cadmium laser provides the LIF excitation source at wavelengths of 325 and 442 nm. Preliminary data are obtained on eight postmortem aorta samples, each 10 mm in length. OCT images and LIF spectra give complementary information from normal and atherosclerotic portions of aorta wall. OCT images show structures such as intima, media, internal elastic lamina, and fibrotic regions. Emission spectra ratios of 520/490 (325-nm excitation) and 595/635 (442-nm excitation) could be used to identify normal and plaque regions with 97 and 91% correct classification rates, respectively. With miniaturization of the delivery probe and improvements in system speed, this dual-modality device could provide a valuable tool for identification and characterization of atherosclerotic plaques.
AB - We develop a dual-modality device that combines the anatomical imaging capabilities of optical coherence tomography (OCT) with the functional capabilities of laser-induced fluorescence (LIF) spectroscopy. OCT provides cross-sectional images of tissue structure to a depth of up to 2 mm with approximately 10-μm resolution. LIF spectroscopy provides histochemical information in the form of emission spectra from a given tissue location. The OCT subsystem utilizes a superluminescent diode with a center wavelength of 1300 nm, whereas a helium cadmium laser provides the LIF excitation source at wavelengths of 325 and 442 nm. Preliminary data are obtained on eight postmortem aorta samples, each 10 mm in length. OCT images and LIF spectra give complementary information from normal and atherosclerotic portions of aorta wall. OCT images show structures such as intima, media, internal elastic lamina, and fibrotic regions. Emission spectra ratios of 520/490 (325-nm excitation) and 595/635 (442-nm excitation) could be used to identify normal and plaque regions with 97 and 91% correct classification rates, respectively. With miniaturization of the delivery probe and improvements in system speed, this dual-modality device could provide a valuable tool for identification and characterization of atherosclerotic plaques.
KW - Aorta
KW - Atherosclerosis
KW - Laser-induced fluorescence
KW - Multimodality
KW - Plaque
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U2 - 10.1117/1.1695564
DO - 10.1117/1.1695564
M3 - Article
C2 - 15189101
AN - SCOPUS:2942756056
SN - 1083-3668
VL - 9
SP - 618
EP - 623
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 3
ER -