TY - JOUR
T1 - Drought re-routes soil microbial carbon metabolism towards emission of volatile metabolites in an artificial tropical rainforest
AU - Honeker, Linnea K.
AU - Pugliese, Giovanni
AU - Ingrisch, Johannes
AU - Fudyma, Jane
AU - Gil-Loaiza, Juliana
AU - Carpenter, Elizabeth
AU - Singer, Esther
AU - Hildebrand, Gina
AU - Shi, Lingling
AU - Hoyt, David W.
AU - Chu, Rosalie K.
AU - Toyoda, Jason
AU - Krechmer, Jordan E.
AU - Claflin, Megan S.
AU - Ayala-Ortiz, Christian
AU - Freire-Zapata, Viviana
AU - Pfannerstill, Eva Y.
AU - Daber, L. Erik
AU - Meeran, Kathiravan
AU - Dippold, Michaela A.
AU - Kreuzwieser, Jürgen
AU - Williams, Jonathan
AU - Ladd, S. Nemiah
AU - Werner, Christiane
AU - Tfaily, Malak M.
AU - Meredith, Laura K.
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/8
Y1 - 2023/8
N2 - Drought impacts on microbial activity can alter soil carbon fate and lead to the loss of stored carbon to the atmosphere as CO2 and volatile organic compounds (VOCs). Here we examined drought impacts on carbon allocation by soil microbes in the Biosphere 2 artificial tropical rainforest by tracking 13C from position-specific 13C-pyruvate into CO2 and VOCs in parallel with multi-omics. During drought, efflux of 13C-enriched acetate, acetone and C4H6O2 (diacetyl) increased. These changes represent increased production and buildup of intermediate metabolites driven by decreased carbon cycling efficiency. Simultaneously,13C-CO2 efflux decreased, driven by a decrease in microbial activity. However, the microbial carbon allocation to energy gain relative to biosynthesis was unchanged, signifying maintained energy demand for biosynthesis of VOCs and other drought-stress-induced pathways. Overall, while carbon loss to the atmosphere via CO2 decreased during drought, carbon loss via efflux of VOCs increased, indicating microbially induced shifts in soil carbon fate.
AB - Drought impacts on microbial activity can alter soil carbon fate and lead to the loss of stored carbon to the atmosphere as CO2 and volatile organic compounds (VOCs). Here we examined drought impacts on carbon allocation by soil microbes in the Biosphere 2 artificial tropical rainforest by tracking 13C from position-specific 13C-pyruvate into CO2 and VOCs in parallel with multi-omics. During drought, efflux of 13C-enriched acetate, acetone and C4H6O2 (diacetyl) increased. These changes represent increased production and buildup of intermediate metabolites driven by decreased carbon cycling efficiency. Simultaneously,13C-CO2 efflux decreased, driven by a decrease in microbial activity. However, the microbial carbon allocation to energy gain relative to biosynthesis was unchanged, signifying maintained energy demand for biosynthesis of VOCs and other drought-stress-induced pathways. Overall, while carbon loss to the atmosphere via CO2 decreased during drought, carbon loss via efflux of VOCs increased, indicating microbially induced shifts in soil carbon fate.
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U2 - 10.1038/s41564-023-01432-9
DO - 10.1038/s41564-023-01432-9
M3 - Article
C2 - 37524975
AN - SCOPUS:85166392000
SN - 2058-5276
VL - 8
SP - 1480
EP - 1494
JO - Nature Microbiology
JF - Nature Microbiology
IS - 8
ER -