TY - JOUR
T1 - Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation
AU - Choudhery, Mahmood S.
AU - Badowski, Michael
AU - Muise, Angela
AU - Pierce, John
AU - Harris, David T.
N1 - Funding Information:
This work was supported, in part, by Adicyte, Inc. The authors also wish to acknowledge our undergraduate students for their help in this study.
PY - 2014/1/7
Y1 - 2014/1/7
N2 - Background: Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism's reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs).Methods: To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16INK4a and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential.Results: Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs.Conclusions: In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.
AB - Background: Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism's reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs).Methods: To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16INK4a and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential.Results: Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs.Conclusions: In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.
KW - Adipose tissue
KW - Donor age
KW - Growth kinetics
KW - In vitro differentiation potential
KW - Mesenchymal stem cells
KW - Regenerative potential
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U2 - 10.1186/1479-5876-12-8
DO - 10.1186/1479-5876-12-8
M3 - Article
C2 - 24397850
AN - SCOPUS:84892147596
SN - 1479-5876
VL - 12
JO - Journal of Translational Medicine
JF - Journal of Translational Medicine
IS - 1
M1 - 8
ER -