TY - JOUR
T1 - Dominant negative rat DNA polymerase β mutants interfere with base excision repair in Saccharomyces cerevisiae
AU - Clairmont, Caroline A.
AU - Sweasy, Joann B.
PY - 1996/2
Y1 - 1996/2
N2 - DNA polymerase β is one of the smallest known eukaryotic DNA polymerases. This polymerase has been very well characterized in vitro, but its functional role in vivo has yet to be determined. Using a novel competition assay in Escherichia coli, we isolated two DNA polymerase β dominant negative mutants. When we overexpressed the dominant negative mutant proteins in Saccharomyces cerevisiae, the cells became sensitive to methyl methanesulfonate. Interestingly, overexpression of the same polymerase β mutant proteins did not confer sensitivity to UV damage, strongly suggesting that the mutant proteins interfere with the process of base excision repair but not nucleotide excision repair in S. cerevisiae. Our data implicate a role for polymerase IV, the S. cerevisiae polymerase β homolog, in base excision repair in S. cerevisiae.
AB - DNA polymerase β is one of the smallest known eukaryotic DNA polymerases. This polymerase has been very well characterized in vitro, but its functional role in vivo has yet to be determined. Using a novel competition assay in Escherichia coli, we isolated two DNA polymerase β dominant negative mutants. When we overexpressed the dominant negative mutant proteins in Saccharomyces cerevisiae, the cells became sensitive to methyl methanesulfonate. Interestingly, overexpression of the same polymerase β mutant proteins did not confer sensitivity to UV damage, strongly suggesting that the mutant proteins interfere with the process of base excision repair but not nucleotide excision repair in S. cerevisiae. Our data implicate a role for polymerase IV, the S. cerevisiae polymerase β homolog, in base excision repair in S. cerevisiae.
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U2 - 10.1128/jb.178.3.656-661.1996
DO - 10.1128/jb.178.3.656-661.1996
M3 - Article
C2 - 8550496
AN - SCOPUS:0030067386
SN - 0021-9193
VL - 178
SP - 656
EP - 661
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 3
ER -