Domain Organization of Chicken Gizzard Myosin Light Chain Kinase Deduced from a Cloned cDNA

Vince Guerriero, Mario A. Russo, Norma J. Olson, Anthony R. Means, John A. Putkey

    Research output: Contribution to journalArticlepeer-review

    98 Scopus citations


    Myosin light chain kinases (MLCK) are the most studied of the calmodulin-activated enzymes; however, minimal sequence information is available for the smooth muscle form of the enzyme. The production of an antibody against the enzyme and the use of expression vectors for constructing cDNA libraries have facilitated the isolation of a cDNA for this kinase. The derived amino sequence was found to contain a region of high homology (54%) to the rabbit skeletal muscle enzyme and also very significant homology (35%) to the catalytic subunit of phosphorylase b kinase and cGMP-dependent protein kinase. All of these homologies were found in the known catalytic domains of these enzyme, thus enabling us to predict the location of the catalytic domain for the chicken gizzard myosin light chain kinase. Within the catalytic domain a consensus sequence for an ATP-binding site was located. Subcloning and expression of different regions of the cDNA defined a 192 base pair fragment coding for the calmodulin-binding domain of MLCK. Both of the cAMP-dependent protein kinase phosphorylation sites were identified by sequence homology. A linear model for MLCK is presented placing the various domains in relative position. Northern blot analysis and S1 protection and mapping experiments have revealed that the mRNA for MLCK is 5.5 kilobases in length, but there also exists a second mRNA of 2.7 kilobases that shares a high degree of homology with about 520 base pairs at the 3' end of the cDNA for MLCK.

    Original languageEnglish (US)
    Pages (from-to)8372-8381
    Number of pages10
    Issue number26
    StatePublished - Dec 1 1986

    ASJC Scopus subject areas

    • Biochemistry


    Dive into the research topics of 'Domain Organization of Chicken Gizzard Myosin Light Chain Kinase Deduced from a Cloned cDNA'. Together they form a unique fingerprint.

    Cite this