DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular‐scale friction

F. Zenhausern; M. Adrian; B. Ten Heggeler‐Bordier; L. M. Eng; P. Descouts

doi:10.1002/sca.4950140405

DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular‐scale friction

F. Zenhausern, M. Adrian, B. Ten Heggeler‐Bordier, L. M. Eng, P. Descouts

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon‐coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA‐covered samples.

Original language	English (US)
Pages (from-to)	212-217
Number of pages	6
Journal	Scanning
Volume	14
Issue number	4
DOIs	https://doi.org/10.1002/sca.4950140405
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Instrumentation

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10.1002/sca.4950140405

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abstract = "Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon‐coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA‐covered samples.",

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T1 - DNA and RNA polymerase/DNA complex imaged by scanning force microscopy

T2 - Influence of molecular‐scale friction

AU - Zenhausern, F.

AU - Adrian, M.

AU - Heggeler‐Bordier, B. Ten

AU - Eng, L. M.

AU - Descouts, P.

PY - 1992

Y1 - 1992

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AB - Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon‐coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA‐covered samples.

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ER -

DNA and RNA polymerase/DNA complex imaged by scanning force microscopy: Influence of molecular‐scale friction

Abstract

ASJC Scopus subject areas

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