TY - JOUR
T1 - Diurnal regulation of plant epidermal wax synthesis through antagonistic roles of the transcription factors SPL9 and DEWAX
AU - Li, Rong Jun
AU - Li, Lin Mao
AU - Liu, Xiu Lin
AU - Kim, Jang Chol
AU - Jenks, Matthew A.
AU - Lu, Shiyou
N1 - Funding Information:
We thank Jia-Wei Wang (Shanghai Institutes for Biological Sciences), Mi ChungSuh(ChonnamNational University), andScott Poethig (University of Pennsylvania) for providing uswith the seeds. This workwas supported by the National Natural Science Foundation of China (NSFC) (31570186 to S.L. and 31400276 to R.-J.L.).
Funding Information:
We thank Jia-Wei Wang (Shanghai Institutes for Biological Sciences), Mi ChungSuh(ChonnamNationalUniversity),andScottPoethig(Universityof Pennsylvania) for providing us with the seeds. This work was supported by the National Natural Science Foundation of China (NSFC) (31570186 to S.L. and 31400276 to R.-J.L.).
Publisher Copyright:
© 2019 ASPB.
PY - 2019/11
Y1 - 2019/11
N2 - Plant surface waxes form an outer barrier that protects the plant from many forms of environmental stress. The deposition of cuticular waxes on the plant surface is regulated by external environmental changes, including light and dark cycles. However, the underlying molecular mechanisms controlling light regulation of wax production are still poorly understood, especially at the posttranscriptional level. In this paper, we report the regulation of cuticular wax production by the miR156- SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9) module in Arabidopsis (Arabidopsis thaliana). When compared with wild-type plants, miR156 and SPL9 mutants showed significantly altered cuticular wax amounts in both stems and leaves. Furthermore, it was found that SPL9 positively regulates gene expression of the alkane-forming enzyme ECERIFERUM1 (CER1), as well as the primary (1-) alcohol-forming enzyme ECERIFERUM4 (CER4), to enhance alkane and 1- alcohol synthesis, respectively. Our results indicate that complex formation of SPL9 with a negative regulator of wax synthesis, DEWAX, will hamper SPL9 DNA binding ability, possibly by interfering with SPL9 homodimerization. Combined with their diurnal gene and protein expressions, this dynamic repression-activation transcriptional module defines a dynamic mechanism that may allow plants to optimize wax synthesis during daily cycles. These findings provide a regulatory framework for environmental signal integration in the regulation of wax synthesis.
AB - Plant surface waxes form an outer barrier that protects the plant from many forms of environmental stress. The deposition of cuticular waxes on the plant surface is regulated by external environmental changes, including light and dark cycles. However, the underlying molecular mechanisms controlling light regulation of wax production are still poorly understood, especially at the posttranscriptional level. In this paper, we report the regulation of cuticular wax production by the miR156- SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9) module in Arabidopsis (Arabidopsis thaliana). When compared with wild-type plants, miR156 and SPL9 mutants showed significantly altered cuticular wax amounts in both stems and leaves. Furthermore, it was found that SPL9 positively regulates gene expression of the alkane-forming enzyme ECERIFERUM1 (CER1), as well as the primary (1-) alcohol-forming enzyme ECERIFERUM4 (CER4), to enhance alkane and 1- alcohol synthesis, respectively. Our results indicate that complex formation of SPL9 with a negative regulator of wax synthesis, DEWAX, will hamper SPL9 DNA binding ability, possibly by interfering with SPL9 homodimerization. Combined with their diurnal gene and protein expressions, this dynamic repression-activation transcriptional module defines a dynamic mechanism that may allow plants to optimize wax synthesis during daily cycles. These findings provide a regulatory framework for environmental signal integration in the regulation of wax synthesis.
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U2 - 10.1105/tpc.19.00233
DO - 10.1105/tpc.19.00233
M3 - Article
C2 - 31484683
AN - SCOPUS:85075095203
SN - 1040-4651
VL - 31
SP - 2711
EP - 2733
JO - Plant Cell
JF - Plant Cell
IS - 11
ER -