Disturbances of amino acids from temporal lobe synaptosomes in human complex partial epilepsy

David M. Labiner; C. C. Yan; Martin E. Weinand; Ryan J. Huxtable

doi:10.1023/A:1022528522373

Disturbances of amino acids from temporal lobe synaptosomes in human complex partial epilepsy

David M. Labiner, C. C. Yan, Martin E. Weinand, Ryan J. Huxtable

Neurosurgery

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We have studied the levels of neuroactive amino acids in synaptosomes (P₂ fraction) isolated from brain tissue of ten patients with medically intractable epilepsy who were undergoing temporal lobectomy. First, lateral temporal tissue (nonfocal) was removed followed by medial temporal tissue (focal). A synaptosomal fraction (P₂) was immediately prepared from each tissue and analyzed for free amino acid concentrations. Statistically significant reductions were seen in glutamine and GABA concentrations in focal tissue compared to nonfocal tissue. The ratio of excitatory amino acids (aspartate and glutamate) to inhibitory amino acids (taurine and GABA) was significantly higher in focal tissue compared to nonfocal. The glutamine/glutamate ratio was significantly reduced. These data support the hypothesis that alterations in the balance between excitatory and inhibitory amino acids may be involved in the expression of epilepsy.

Original language	English (US)
Pages (from-to)	1379-1383
Number of pages	5
Journal	Neurochemical Research
Volume	24
Issue number	11
DOIs	https://doi.org/10.1023/A:1022528522373
State	Published - 1999

Keywords

Amino acids
Epilepsy
GABA
Glutamate
Glutamine
Synaptosomes
Taurine

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1023/A:1022528522373

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abstract = "We have studied the levels of neuroactive amino acids in synaptosomes (P2 fraction) isolated from brain tissue of ten patients with medically intractable epilepsy who were undergoing temporal lobectomy. First, lateral temporal tissue (nonfocal) was removed followed by medial temporal tissue (focal). A synaptosomal fraction (P2) was immediately prepared from each tissue and analyzed for free amino acid concentrations. Statistically significant reductions were seen in glutamine and GABA concentrations in focal tissue compared to nonfocal tissue. The ratio of excitatory amino acids (aspartate and glutamate) to inhibitory amino acids (taurine and GABA) was significantly higher in focal tissue compared to nonfocal. The glutamine/glutamate ratio was significantly reduced. These data support the hypothesis that alterations in the balance between excitatory and inhibitory amino acids may be involved in the expression of epilepsy.",

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Disturbances of amino acids from temporal lobe synaptosomes in human complex partial epilepsy

Abstract

Keywords

ASJC Scopus subject areas

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