Distribution of NMDAR1 receptor subunit mRNA and [125I]MK-801 binding in the hypothalamus of intact, castrate and castrate-DHTP treated male rats

Laura Kus; Robert J. Handa; Jennifer J. Sanderson; Janice E. Kerr; Alvin J. Beitz

doi:10.1016/0169-328X(94)00179-I

Distribution of NMDAR1 receptor subunit mRNA and [¹²⁵I]MK-801 binding in the hypothalamus of intact, castrate and castrate-DHTP treated male rats

Laura Kus, Robert J. Handa, Jennifer J. Sanderson, Janice E. Kerr, Alvin J. Beitz

Basic Medical Sciences, Phoenix

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

This study examines NMDAR1 receptor subunit mRNA expression and [¹²⁵I]MK-801 binding in hypothalamic and limbic nuclei of intact, castrate and castrate-dihydrotestosterone propionate (DHTP)-treated male rats. In intact rats, the highest levels of NMDAR1 mRNA were observed in the supraoptic, suprachiasmatic, ventromedial and arcuate nuclei. Low levels of hybridization were observed in the bed nucleus of the stria terminalis, lateral preoptic area, lateral hypothalamic area and lateral septum. In castrated rats both NMDAR1 mRNA and [¹²⁵I]MK-801 binding are significantly decreased in the lateral septum compared to castrate rats treated with DHTP, a non-aromatizable androgen. NMDAR1 mRNA was also significantly decreased in the supraoptic nucleus of castrate rats when compared to castrate rats treated with DHTP. These data suggest that androgens may modulate NMDA receptor function in some parts of the central nervous system.

Original language	English (US)
Pages (from-to)	55-60
Number of pages	6
Journal	Molecular Brain Research
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(94)00179-I
State	Published - Jan 1995

Keywords

Androgen
Castration
Excitatory amino acid
Glutamate
Hypothalamus
Lateral septum
MK-801
NMDA
NMDAR1
Supraoptic nucleus
mRNA

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0169-328X(94)00179-I

Cite this

@article{02ce27ade67f4e298fd7614281caed0d,

title = "Distribution of NMDAR1 receptor subunit mRNA and [125I]MK-801 binding in the hypothalamus of intact, castrate and castrate-DHTP treated male rats",

abstract = "This study examines NMDAR1 receptor subunit mRNA expression and [125I]MK-801 binding in hypothalamic and limbic nuclei of intact, castrate and castrate-dihydrotestosterone propionate (DHTP)-treated male rats. In intact rats, the highest levels of NMDAR1 mRNA were observed in the supraoptic, suprachiasmatic, ventromedial and arcuate nuclei. Low levels of hybridization were observed in the bed nucleus of the stria terminalis, lateral preoptic area, lateral hypothalamic area and lateral septum. In castrated rats both NMDAR1 mRNA and [125I]MK-801 binding are significantly decreased in the lateral septum compared to castrate rats treated with DHTP, a non-aromatizable androgen. NMDAR1 mRNA was also significantly decreased in the supraoptic nucleus of castrate rats when compared to castrate rats treated with DHTP. These data suggest that androgens may modulate NMDA receptor function in some parts of the central nervous system.",

keywords = "Androgen, Castration, Excitatory amino acid, Glutamate, Hypothalamus, Lateral septum, MK-801, NMDA, NMDAR1, Supraoptic nucleus, mRNA",

author = "Laura Kus and Handa, {Robert J.} and Sanderson, {Jennifer J.} and Kerr, {Janice E.} and Beitz, {Alvin J.}",

note = "Funding Information: We thank Lori Wellman for her assistancew ith photographayn d Vicki King and Joan Hautman for their assistancew ith statisticala nalysiso f the data. This work was supportedb y NIH Grants DA 06687 (A.J.B.), DE 06682 (A.J.B.), NS 31318 (A.J.B.), AA08696( R.J.H.) and NSF BNS 9109226(R .J.H.).",

year = "1995",

month = jan,

doi = "10.1016/0169-328X(94)00179-I",

language = "English (US)",

volume = "28",

pages = "55--60",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Distribution of NMDAR1 receptor subunit mRNA and [125I]MK-801 binding in the hypothalamus of intact, castrate and castrate-DHTP treated male rats

AU - Kus, Laura

AU - Handa, Robert J.

AU - Sanderson, Jennifer J.

AU - Kerr, Janice E.

AU - Beitz, Alvin J.

N1 - Funding Information: We thank Lori Wellman for her assistancew ith photographayn d Vicki King and Joan Hautman for their assistancew ith statisticala nalysiso f the data. This work was supportedb y NIH Grants DA 06687 (A.J.B.), DE 06682 (A.J.B.), NS 31318 (A.J.B.), AA08696( R.J.H.) and NSF BNS 9109226(R .J.H.).

PY - 1995/1

Y1 - 1995/1

N2 - This study examines NMDAR1 receptor subunit mRNA expression and [125I]MK-801 binding in hypothalamic and limbic nuclei of intact, castrate and castrate-dihydrotestosterone propionate (DHTP)-treated male rats. In intact rats, the highest levels of NMDAR1 mRNA were observed in the supraoptic, suprachiasmatic, ventromedial and arcuate nuclei. Low levels of hybridization were observed in the bed nucleus of the stria terminalis, lateral preoptic area, lateral hypothalamic area and lateral septum. In castrated rats both NMDAR1 mRNA and [125I]MK-801 binding are significantly decreased in the lateral septum compared to castrate rats treated with DHTP, a non-aromatizable androgen. NMDAR1 mRNA was also significantly decreased in the supraoptic nucleus of castrate rats when compared to castrate rats treated with DHTP. These data suggest that androgens may modulate NMDA receptor function in some parts of the central nervous system.

AB - This study examines NMDAR1 receptor subunit mRNA expression and [125I]MK-801 binding in hypothalamic and limbic nuclei of intact, castrate and castrate-dihydrotestosterone propionate (DHTP)-treated male rats. In intact rats, the highest levels of NMDAR1 mRNA were observed in the supraoptic, suprachiasmatic, ventromedial and arcuate nuclei. Low levels of hybridization were observed in the bed nucleus of the stria terminalis, lateral preoptic area, lateral hypothalamic area and lateral septum. In castrated rats both NMDAR1 mRNA and [125I]MK-801 binding are significantly decreased in the lateral septum compared to castrate rats treated with DHTP, a non-aromatizable androgen. NMDAR1 mRNA was also significantly decreased in the supraoptic nucleus of castrate rats when compared to castrate rats treated with DHTP. These data suggest that androgens may modulate NMDA receptor function in some parts of the central nervous system.

KW - Androgen

KW - Castration

KW - Excitatory amino acid

KW - Glutamate

KW - Hypothalamus

KW - Lateral septum

KW - MK-801

KW - NMDA

KW - NMDAR1

KW - Supraoptic nucleus

KW - mRNA

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U2 - 10.1016/0169-328X(94)00179-I

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AN - SCOPUS:0028861283

SN - 0169-328X

VL - 28

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JO - Molecular Brain Research

JF - Molecular Brain Research

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