Dissociation of brain tissue into viable single neurons in a microfluidic device

Linan Jiang; Robert Kraft; Linda L. Restifo; Yitshak Zohar

doi:10.1109/NANOMED.2015.7492500

Dissociation of brain tissue into viable single neurons in a microfluidic device

Linan Jiang, Robert Kraft, Linda L. Restifo, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

A microfluidic technology-based tissue-dissociation device has for the first time been designed, fabricated and characterized for the purpose of primary neuronal cell culture. The system has been utilized for controlled dissociation, under an oscillatory flow field, of freshly explanted, enzyme-treated Drosophila larval central nervous system (CNS) into individual, viable neurons capable of robust outgrowth during in vitro culture. Device dimensions, constriction height and width, and operating conditions, flow-rate amplitude and frequency, have been determined based on video microscopy as well as quantitative analyses of the subsequent neuron-culture results.

Original language	English (US)
Title of host publication	9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
Publisher	IEEE Computer Society
Pages	29-32
Number of pages	4
ISBN (Electronic)	9781467396714
DOIs	https://doi.org/10.1109/NANOMED.2015.7492500
State	Published - Jun 15 2016
Event	9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 - Honolulu, United States Duration: Nov 15 2015 → Nov 18 2015

Publication series

Name	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume	2016-June
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Other

Other	9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
Country/Territory	United States
City	Honolulu
Period	11/15/15 → 11/18/15

Keywords

brain tissue dissociation
microfluidic device
neuron culture
oscillating flow field
single neurons

ASJC Scopus subject areas

Molecular Medicine
Biomedical Engineering
Microbiology (medical)

Access to Document

10.1109/NANOMED.2015.7492500

Cite this

Jiang, L., Kraft, R., Restifo, L. L., & Zohar, Y. (2016). Dissociation of brain tissue into viable single neurons in a microfluidic device. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 (pp. 29-32). Article 7492500 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2016-June). IEEE Computer Society. https://doi.org/10.1109/NANOMED.2015.7492500

Dissociation of brain tissue into viable single neurons in a microfluidic device. / Jiang, Linan; Kraft, Robert; Restifo, Linda L. et al.
9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. IEEE Computer Society, 2016. p. 29-32 7492500 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2016-June).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Jiang, L, Kraft, R, Restifo, LL & Zohar, Y 2016, Dissociation of brain tissue into viable single neurons in a microfluidic device. in 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015., 7492500, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, vol. 2016-June, IEEE Computer Society, pp. 29-32, 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015, Honolulu, United States, 11/15/15. https://doi.org/10.1109/NANOMED.2015.7492500

Jiang L, Kraft R, Restifo LL , Zohar Y. Dissociation of brain tissue into viable single neurons in a microfluidic device. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. IEEE Computer Society. 2016. p. 29-32. 7492500. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). doi: 10.1109/NANOMED.2015.7492500

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Dissociation of brain tissue into viable single neurons in a microfluidic device

Abstract

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Keywords

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