Disruption of TP63-miR-27a* feedback loop by mutant TP53 in head and neck cancer

Nikhil S. Chari, Cristina Ivan, Xiandong Le, Jinzhong Li, Ainiwaer Mijiti, Ameeta A. Patel, Abdullah A. Osman, Christine B. Peterson, Michelle D. Williams, Curtis R. Pickering, Carlos Caulin, Jeffrey N. Myers, George A. Calin, Stephen Y. Lai

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background: Alterations in the epidermal growth factor receptor and PI3K pathways in head and neck squamous cell carcinomas (HNSCCs) are frequent events that promote tumor progression. Ectopic expression of the epidermal growth factor receptor-targeting microRNA (miR), miR-27a* (miR-27a-5p), inhibits tumor growth. We sought to identify mechanisms mediating repression of miR-27a* in HNSCC, which have not been previously identified. Methods: We quantified miR-27a* in 47 oral cavity squamous cell carcinoma patient samples along with analysis of miR-27a* in 73 oropharyngeal and 66 human papillomavirus-positive (HPVþ) samples from The Cancer Genome Atlas. In vivo and in vitro TP53 models engineered to express mutant TP53, along with promoter analysis using chromatin immunoprecipitation and luciferase assays, were used to identify the role of TP53 and TP63 in miR-27a* transcription. An HNSCC cell line engineered to conditionally express miR-27a* was used in vitro to determine effects of miR-27a* on target genes and tumor cells. Results: miR-27a* expression was repressed in 47 oral cavity tumor samples vs matched normal tissue (mean log2 difference ¼ -0.023, 95% confidence interval ¼ -0.044 to -0.002; two-sided paired t test, P ¼.03), and low miR-27a* levels were associated with poor survival in HPVþ and oropharyngeal HNSCC samples. Binding of DNp63a to the promoter led to an upregulation of miR-27a*. In vitro and in vivo findings showed that mutant TP53 represses the miR-27a* promoter, downregulating miR-27a* levels. DNp63a and nucleoporin 62, a protein involved in DNP63a transport, were validated as novel targets of miR-27a*. Conclusion: Our results characterize a negative feedback loop between TP63 and miR-27a*. Genetic alterations in TP53, a frequent event in HNSCC, disrupt this regulatory loop by repressing miR-27a* expression, promoting tumor survival.

Original languageEnglish (US)
Article numberDJZ097
Pages (from-to)266-277
Number of pages12
JournalJournal of the National Cancer Institute
Issue number3
StatePublished - 2021

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


Dive into the research topics of 'Disruption of TP63-miR-27a* feedback loop by mutant TP53 in head and neck cancer'. Together they form a unique fingerprint.

Cite this