Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Aldemar Montero; Yosuke Okada; Masaro Tomita; Masako Ito; Hiroshi Tsurukami; Toshitaka Nakamura; Thomas Doetschman; J. Douglas Coffin; Marja M. Hurley

doi:10.1172/JCI8641

Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Aldemar Montero, Yosuke Okada, Masaro Tomita, Masako Ito, Hiroshi Tsurukami, Toshitaka Nakamura, Thomas Doetschman, J. Douglas Coffin, Marja M. Hurley

Research output: Contribution to journal › Article › peer-review

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Abstract

Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

Original language	English (US)
Pages (from-to)	1085-1093
Number of pages	9
Journal	Journal of Clinical Investigation
Volume	105
Issue number	8
DOIs	https://doi.org/10.1172/JCI8641
State	Published - Apr 2000
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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10.1172/JCI8641

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title = "Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation",

abstract = "Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.",

author = "Aldemar Montero and Yosuke Okada and Masaro Tomita and Masako Ito and Hiroshi Tsurukami and Toshitaka Nakamura and Thomas Doetschman and Coffin, {J. Douglas} and Hurley, {Marja M.}",

year = "2000",

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AU - Montero, Aldemar

AU - Okada, Yosuke

AU - Tomita, Masaro

AU - Ito, Masako

AU - Tsurukami, Hiroshi

AU - Nakamura, Toshitaka

AU - Doetschman, Thomas

AU - Coffin, J. Douglas

AU - Hurley, Marja M.

PY - 2000/4

Y1 - 2000/4

N2 - Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

AB - Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

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Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Abstract

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