Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer

Jian Shi; Yifan Wang; Lei Zeng; Yadi Wu; Jiong Deng; Qiang Zhang; Yiwei Lin; Junlin Li; Tiebang Kang; Min Tao; Elena Rusinova; Guangtao Zhang; Chi Wang; Haining Zhu; Jun Yao; Yi Xin Zeng; B. Mark Evers; Ming Ming Zhou; Binhua P. Zhou

doi:10.1016/j.ccr.2014.01.028

Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer

Jian Shi
, Yifan Wang
, Lei Zeng
, Yadi Wu
, Jiong Deng
, Qiang Zhang
, Yiwei Lin
, Junlin Li
, Tiebang Kang
, Min Tao
, Elena Rusinova
, Guangtao Zhang
, Chi Wang
, Haining Zhu
, Jun Yao
, Yi Xin Zeng
, B. Mark Evers
, Ming Ming Zhou
, Binhua P. Zhou

Research output: Contribution to journal › Article › peer-review

427 Scopus citations

Abstract

Twist is a key transcription activator of epithelial-mesenchymal transition (EMT). It remains unclear how Twist induces gene expression. Here we report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Twist contains a "histone H4-mimic" GK-X-GK motif that is diacetylated by Tip60. The diacetylated Twist binds the second bromodomain of BRD4, whose first bromodomain interacts with acetylated H4, thereby constructing an activated Twist/BRD4/P-TEFb/RNA-Pol II complex at the WNT5A promoter and enhancer. Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell (CSC)-like properties, and tumorigenicity of BLBC cells. Our study indicates that the interaction with BRD4 is critical for the oncogenic function of Twist in BLBC.

Original language	English (US)
Pages (from-to)	210-225
Number of pages	16
Journal	Cancer Cell
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/j.ccr.2014.01.028
State	Published - Feb 10 2014
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.ccr.2014.01.028

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Shi, J., Wang, Y., Zeng, L., Wu, Y., Deng, J., Zhang, Q., Lin, Y., Li, J., Kang, T., Tao, M., Rusinova, E., Zhang, G., Wang, C., Zhu, H., Yao, J., Zeng, Y. X., Evers, B. M., Zhou, M. M., & Zhou, B. P. (2014). Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer. Cancer Cell, 25(2), 210-225. https://doi.org/10.1016/j.ccr.2014.01.028

Shi, J, Wang, Y, Zeng, L, Wu, Y, Deng, J, Zhang, Q, Lin, Y, Li, J, Kang, T, Tao, M, Rusinova, E, Zhang, G, Wang, C, Zhu, H, Yao, J, Zeng, YX, Evers, BM, Zhou, MM & Zhou, BP 2014, 'Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer', Cancer Cell, vol. 25, no. 2, pp. 210-225. https://doi.org/10.1016/j.ccr.2014.01.028

@article{485f96431a43456997fe422fb5c7ba30,

title = "Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer",

abstract = "Twist is a key transcription activator of epithelial-mesenchymal transition (EMT). It remains unclear how Twist induces gene expression. Here we report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Twist contains a {"}histone H4-mimic{"} GK-X-GK motif that is diacetylated by Tip60. The diacetylated Twist binds the second bromodomain of BRD4, whose first bromodomain interacts with acetylated H4, thereby constructing an activated Twist/BRD4/P-TEFb/RNA-Pol II complex at the WNT5A promoter and enhancer. Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell (CSC)-like properties, and tumorigenicity of BLBC cells. Our study indicates that the interaction with BRD4 is critical for the oncogenic function of Twist in BLBC.",

author = "Jian Shi and Yifan Wang and Lei Zeng and Yadi Wu and Jiong Deng and Qiang Zhang and Yiwei Lin and Junlin Li and Tiebang Kang and Min Tao and Elena Rusinova and Guangtao Zhang and Chi Wang and Haining Zhu and Jun Yao and Zeng, \{Yi Xin\} and Evers, \{B. Mark\} and Zhou, \{Ming Ming\} and Zhou, \{Binhua P.\}",

note = "Funding Information: We thank Cathy Anthony for critical reading and editing of this manuscript. We also thank Dr. Jing Chen for technical assistance on mass spectrometry analysis. We are grateful to Dr. James E. Bradner for providing JQ1, Dr. Robert A. Weinberg for HMLE cells, Dr. Michael Rosenblatt for SUM1315 cells, and Dr. Bruno Amati for Tip60 antibodies as valuable reagents for this study. This work was supported in part by grants from the National Institute of Health (CA125454 to B.P.Z., P20CA153043 to B.M.E., and HG004508 and CA87658 to M.-M.Z.), the American Cancer Society (RSG13187 to Y.W.), and the Nature Science Foundation of China (91129303 to J.D.). We acknowledge the University of Kentucky Proteomics Core, which is partially supported by grants from the National Cancer Institute (P30CA177558) and the National Institute of General Medical Sciences (P20GM103486). ",

year = "2014",

month = feb,

day = "10",

doi = "10.1016/j.ccr.2014.01.028",

language = "English (US)",

volume = "25",

pages = "210--225",

journal = "Cancer Cell",

issn = "1535-6108",

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T1 - Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer

AU - Shi, Jian

AU - Wang, Yifan

AU - Zeng, Lei

AU - Wu, Yadi

AU - Deng, Jiong

AU - Zhang, Qiang

AU - Lin, Yiwei

AU - Li, Junlin

AU - Kang, Tiebang

AU - Tao, Min

AU - Rusinova, Elena

AU - Zhang, Guangtao

AU - Wang, Chi

AU - Zhu, Haining

AU - Yao, Jun

AU - Zeng, Yi Xin

AU - Evers, B. Mark

AU - Zhou, Ming Ming

AU - Zhou, Binhua P.

N1 - Funding Information: We thank Cathy Anthony for critical reading and editing of this manuscript. We also thank Dr. Jing Chen for technical assistance on mass spectrometry analysis. We are grateful to Dr. James E. Bradner for providing JQ1, Dr. Robert A. Weinberg for HMLE cells, Dr. Michael Rosenblatt for SUM1315 cells, and Dr. Bruno Amati for Tip60 antibodies as valuable reagents for this study. This work was supported in part by grants from the National Institute of Health (CA125454 to B.P.Z., P20CA153043 to B.M.E., and HG004508 and CA87658 to M.-M.Z.), the American Cancer Society (RSG13187 to Y.W.), and the Nature Science Foundation of China (91129303 to J.D.). We acknowledge the University of Kentucky Proteomics Core, which is partially supported by grants from the National Cancer Institute (P30CA177558) and the National Institute of General Medical Sciences (P20GM103486).

PY - 2014/2/10

Y1 - 2014/2/10

N2 - Twist is a key transcription activator of epithelial-mesenchymal transition (EMT). It remains unclear how Twist induces gene expression. Here we report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Twist contains a "histone H4-mimic" GK-X-GK motif that is diacetylated by Tip60. The diacetylated Twist binds the second bromodomain of BRD4, whose first bromodomain interacts with acetylated H4, thereby constructing an activated Twist/BRD4/P-TEFb/RNA-Pol II complex at the WNT5A promoter and enhancer. Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell (CSC)-like properties, and tumorigenicity of BLBC cells. Our study indicates that the interaction with BRD4 is critical for the oncogenic function of Twist in BLBC.

AB - Twist is a key transcription activator of epithelial-mesenchymal transition (EMT). It remains unclear how Twist induces gene expression. Here we report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Twist contains a "histone H4-mimic" GK-X-GK motif that is diacetylated by Tip60. The diacetylated Twist binds the second bromodomain of BRD4, whose first bromodomain interacts with acetylated H4, thereby constructing an activated Twist/BRD4/P-TEFb/RNA-Pol II complex at the WNT5A promoter and enhancer. Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell (CSC)-like properties, and tumorigenicity of BLBC cells. Our study indicates that the interaction with BRD4 is critical for the oncogenic function of Twist in BLBC.

UR - https://www.scopus.com/pages/publications/84893519959

UR - https://www.scopus.com/pages/publications/84893519959#tab=citedBy

U2 - 10.1016/j.ccr.2014.01.028

DO - 10.1016/j.ccr.2014.01.028

M3 - Article

C2 - 24525235

AN - SCOPUS:84893519959

SN - 1535-6108

VL - 25

SP - 210

EP - 225

JO - Cancer Cell

JF - Cancer Cell

IS - 2

ER -

Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-like Breast Cancer

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