Discordant inheritance of mitochondrial and plastid DNA in diverse alfalfa genotypes

N. R. Forsthoefel; H. J. Bohnert; S. E. Smith

doi:10.1093/oxfordjournals.jhered.a111228

Discordant inheritance of mitochondrial and plastid DNA in diverse alfalfa genotypes

N. R. Forsthoefel, H. J. Bohnert, S. E. Smith

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Abstract

Previous research has established that plastids are inherited biparentally in alfalfa (Medicago sativa L.), whereas mitochondrial DNA (mtDNA) has been shown in limited studies to be inherited in a uniparental-maternal fashion. However, ultrastructural and genetic evidence suggests that mitochondria could be inherited from the paternal parent in at least some alfalfa genotypes. This study investigated the inheritance of mtDNA in 33 F₁ progenies from crosses between two cytoplasmic male sterile maternal plants and 25 paternal plants. The paternal parents represented each of the eight basic germplasm groups of M. sativa. Cloned mtDNA fragments from M. sativa were used as hybridization probes to identify polymorphisms in mtDNA for Eco RI restriction sites. Polymorphisms were compared in parents and individual progenies to determine patterns of mtDNA inheritance. All progenies displayed the restriction pattern of the maternal parent, indicating that paternal inheritance of mtDNA in alfalfa is at best rare. A portion of the same progenies (N = 17) were also examined for their chloroplast DNA inheritance patterns. Chloroplast DNA displayed a mixed inheritance pattern, with six progenies showing uniparental-maternal and 11 progenies uniparental-paternal inheritance.

Original language	English (US)
Pages (from-to)	342-345
Number of pages	4
Journal	Journal of Heredity
Volume	83
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.jhered.a111228
State	Published - Sep 1992

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Genetics
Genetics(clinical)

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title = "Discordant inheritance of mitochondrial and plastid DNA in diverse alfalfa genotypes",

abstract = "Previous research has established that plastids are inherited biparentally in alfalfa (Medicago sativa L.), whereas mitochondrial DNA (mtDNA) has been shown in limited studies to be inherited in a uniparental-maternal fashion. However, ultrastructural and genetic evidence suggests that mitochondria could be inherited from the paternal parent in at least some alfalfa genotypes. This study investigated the inheritance of mtDNA in 33 F1 progenies from crosses between two cytoplasmic male sterile maternal plants and 25 paternal plants. The paternal parents represented each of the eight basic germplasm groups of M. sativa. Cloned mtDNA fragments from M. sativa were used as hybridization probes to identify polymorphisms in mtDNA for Eco RI restriction sites. Polymorphisms were compared in parents and individual progenies to determine patterns of mtDNA inheritance. All progenies displayed the restriction pattern of the maternal parent, indicating that paternal inheritance of mtDNA in alfalfa is at best rare. A portion of the same progenies (N = 17) were also examined for their chloroplast DNA inheritance patterns. Chloroplast DNA displayed a mixed inheritance pattern, with six progenies showing uniparental-maternal and 11 progenies uniparental-paternal inheritance.",

author = "Forsthoefel, {N. R.} and Bohnert, {H. J.} and Smith, {S. E.}",

note = "Funding Information: From the Departments of Plant Sciences (Forsthoefel, Bohnert, and Smith) and Biochemistry (Bohnert), University of Arizona, Tucson, AZ 85721. This research was supported by the Arizona Agricultural Experiment Station. The authors thank Gabriel Meyer for her excellent technical assistance and H. L. Mogensen for his guidance and support during the planning and execution of this research. Address reprint requests to S. E. Smith at the address above.",

year = "1992",

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doi = "10.1093/oxfordjournals.jhered.a111228",

language = "English (US)",

volume = "83",

pages = "342--345",

journal = "Journal of Heredity",

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AU - Forsthoefel, N. R.

AU - Bohnert, H. J.

AU - Smith, S. E.

N1 - Funding Information: From the Departments of Plant Sciences (Forsthoefel, Bohnert, and Smith) and Biochemistry (Bohnert), University of Arizona, Tucson, AZ 85721. This research was supported by the Arizona Agricultural Experiment Station. The authors thank Gabriel Meyer for her excellent technical assistance and H. L. Mogensen for his guidance and support during the planning and execution of this research. Address reprint requests to S. E. Smith at the address above.

PY - 1992/9

Y1 - 1992/9

N2 - Previous research has established that plastids are inherited biparentally in alfalfa (Medicago sativa L.), whereas mitochondrial DNA (mtDNA) has been shown in limited studies to be inherited in a uniparental-maternal fashion. However, ultrastructural and genetic evidence suggests that mitochondria could be inherited from the paternal parent in at least some alfalfa genotypes. This study investigated the inheritance of mtDNA in 33 F1 progenies from crosses between two cytoplasmic male sterile maternal plants and 25 paternal plants. The paternal parents represented each of the eight basic germplasm groups of M. sativa. Cloned mtDNA fragments from M. sativa were used as hybridization probes to identify polymorphisms in mtDNA for Eco RI restriction sites. Polymorphisms were compared in parents and individual progenies to determine patterns of mtDNA inheritance. All progenies displayed the restriction pattern of the maternal parent, indicating that paternal inheritance of mtDNA in alfalfa is at best rare. A portion of the same progenies (N = 17) were also examined for their chloroplast DNA inheritance patterns. Chloroplast DNA displayed a mixed inheritance pattern, with six progenies showing uniparental-maternal and 11 progenies uniparental-paternal inheritance.

AB - Previous research has established that plastids are inherited biparentally in alfalfa (Medicago sativa L.), whereas mitochondrial DNA (mtDNA) has been shown in limited studies to be inherited in a uniparental-maternal fashion. However, ultrastructural and genetic evidence suggests that mitochondria could be inherited from the paternal parent in at least some alfalfa genotypes. This study investigated the inheritance of mtDNA in 33 F1 progenies from crosses between two cytoplasmic male sterile maternal plants and 25 paternal plants. The paternal parents represented each of the eight basic germplasm groups of M. sativa. Cloned mtDNA fragments from M. sativa were used as hybridization probes to identify polymorphisms in mtDNA for Eco RI restriction sites. Polymorphisms were compared in parents and individual progenies to determine patterns of mtDNA inheritance. All progenies displayed the restriction pattern of the maternal parent, indicating that paternal inheritance of mtDNA in alfalfa is at best rare. A portion of the same progenies (N = 17) were also examined for their chloroplast DNA inheritance patterns. Chloroplast DNA displayed a mixed inheritance pattern, with six progenies showing uniparental-maternal and 11 progenies uniparental-paternal inheritance.

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Discordant inheritance of mitochondrial and plastid DNA in diverse alfalfa genotypes

Abstract

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