Direct peritoneal resuscitation from hemorrhagic shock: Effect of time delay in therapy initiation

El Rasheid Zakaria, R. Neal Garrison, Touichi Kawabe, Patrick D. Harris, Carl J. Hauser, Rao R. Ivatury, H. Gill Cryer, Slate Wilson, R. Neal Garrison

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Background: After conventional resuscitation from hemorrhagic shock, splanchnic microvessels progressively constrict, leading to impairment of blood flow. This occurs despite restoration and maintenance of central hemodynamics. The authors' recent studies have demonstrated that topical and continuous ex vivo exposure of the gut microvasculature to a glucose-based clinical peritoneal dialysis solution (Delflex), as a technique of direct peritoneal resuscitation (DPR), can prevent these postresuscitation events when initiated simultaneously with conventional resuscitation. This study aimed to determine whether DPR applied after conventional resuscitation reverses the established postresuscitation intestinal vasoconstriction and hypoperfusion. Methods: Male Sprague-Dawley rats were bled to 50% of baseline mean arterial pressure and resuscitated intravenously over 30 minutes with the shed blood returned plus two times the shed blood volume of saline. Initiation of ex vivo, topical DPR was delayed to 2 hours (group 1, n = 8), or to 4 hours (group 2, n = 8), respectively, after conventional resuscitation. Intravital microscopy and Doppler velocimetry were used to measure terminal ileal microvascular diameters of inflow A1 and premucosal A3 (proximal pA3, distal dA3) arterioles and blood flow in the Al arteriole, respectively. Maximum arteriolar dilation capacity was obtained from the topical application, in the tissue bath, of the endothelium-independent nitric oxide-donor sodium nitroprusside (10 -4M). Results: Hemorrhagic shock caused a selective vasoconstriction of A1 (-24.1% ± 2.15%) arterioles from baseline, which was not seen in A3 vessels. This caused Al blood flow to drop by -68.6% of the prehemorrhage value. Conventional resuscitation restored and maintained hemodynamics in all the animals without additional fluid therapy. In contrast, there was a generalized and progressive postresuscitation vasoconstriction of A1 (-21.7%), pA3 (-18.5%), and dA3 (-18.7%) vessels. The average postresuscitation A1 blood flow was -49.5% of the prehemorrhage value, indicating a persistent postresuscitation hypoperfusion. Direct peritoneal resuscitation reversed the postresuscitation vasoconstriction by 40.9% and enhanced A1 blood flow by 112.9% of the respective postresuscitation values. Conclusions: Delayed DPR reverses the gut postresuscitation vasoconstriction and hypoperfusion regardless of the initiation time. This occurs without adverse effects on hemodynamics. Direct peritoneal resuscitation-mediated enhancement of tissue perfusion results from the local effects from the vasoactive components of the Delflex solution, which are hyperosmolality, lactate buffer anion, and, to a lesser extent, low pH. The molecular mechanism of this vasodilation effect needs further investigation.

Original languageEnglish (US)
Pages (from-to)499-508
Number of pages10
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume58
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • Hemorrhagic shock
  • Hyperosmolality
  • Peritoneal therapy
  • Resuscitation
  • Trauma care

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

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