@inproceedings{fd71c6f6f3fb4068bca05de6f2dbca28,
title = "Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM)",
abstract = "Bloodstream infection (BSI), an expensive and deadly condition, burdens healthcare. Improved BSI treatment relies on rapid, point-of-care diagnosis of causative species and presence of plasmid-mediated drug resistance. We have designed, fabricated and assembled an apparatus for automated PCR amplification by wire-guided droplet manipulation (WDM). WDM minimizes thermal resistance and exploits convective heat transfer to increase reaction speeds. Molecular partitioning within the water-in-oil droplet enables PCR amplification with whole blood (WB) in situ. The V3 region of the 16s rRNA gene and a representative 4.884 kb plasmid were amplified by WDM from 10% WB. Optics has been designed for real-time detection.",
keywords = "16s rRNA, Bloodstream infection, Molecular partitioning, PCR",
author = "Harshman, {Dustin K.} and Roberto Reyes and Yoon, {Jeong Yeol}",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "470--472",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}