Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins

Kendra D. Marr, Natalia A. Ignatenko, Noel A. Warfel, Ken Batai, Anne E. Cress, Grant R. Pollock, Ava C. Wong, Benjamin R. Lee

Research output: Contribution to journalArticlepeer-review

Abstract

The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate via time-lapse imaging distinct phenotypes of prostate organoids derived from patient material– without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p<0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology.

Original languageEnglish (US)
Article number1083150
JournalFrontiers in Oncology
Volume12
DOIs
StatePublished - Jan 13 2023

Keywords

  • cancer phenotypes
  • extracellular matrix
  • organoids
  • prostate cancer
  • video microscopy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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