Differential response of BEAS-2B and H-441 cells to methylene blue photoactivation

Rosalva Josefina Rodríguez-Córdova, Cindy Alejandra Gutiérrez-Valenzuela, Pasano Bojang, Reynaldo Esquivel, Pedro Hernández, Kenneth Ramos, Roberto Guzmán-Zamudio, Armando Lucero-Acuña

Research output: Contribution to journalArticlepeer-review


Background/Aim: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441). Materials and Methods: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay. Results: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control. Conclusion: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.

Original languageEnglish (US)
Pages (from-to)3739-3744
Number of pages6
JournalAnticancer research
Issue number7
StatePublished - 2019


  • Lung cancer
  • Methylene blue
  • Photodynamic therapy
  • ROS production

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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