Differential effects of EGF on repair of cellular functions after dichlorovinyl-L-cysteine-induced injury

Grazyna Nowak, Kenneth B. Keasler, Douglas E. McKeller, Rick G. Schnellmann

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


This study examined the repair of renal proximal tubule cellular (RPTC) functions following sublethal injury induced by the nephrotoxicant S-(1,2- dichlorovinyl)-L-cysteine (DCVC). DCVC exposure resulted in 31% cell death and loss 24 h following the treatment. Monolayer confluence recovered through migration/spreading but not proliferation after 6 days. Basal, uncoupled, and ouabain-sensitive oxygen consumption (QO2) decreased 47, 76, and 62%, respectively, 24 h after DCVC exposure. Na+-K+-ATPase activity and Na+- dependent glucose uptake were inhibited 80 and 68%, respectively, 24 h after DCVC exposure. None of these functions recovered over time. Addition of epidermal growth factor (EGF) following DCVC exposure did not prevent decreases in basal, uncoupled, and ouabain-sensitive QO2 values and Na+- K+-ATPase activity but promoted their recovery over 4-6 days. In contrast, no recovery of Na+-dependent glucose uptake occurred in the presence of EGF. These data show that: 1) DCVC exposure decreases mitochondrial function, Na+-K+-ATPase activity, active Na+ transport, and Na+-dependent glucose uptake in sublethally injured RPTC; 2) DCVC-treated RPTC do not proliferate nor regain their physiological functions in this model; and 3) EGF promotes recovery of mitochondrial function and active Na+ transport but not Na+- dependent glucose uptake. These results suggest that cysteine conjugates may cause renal dysfunction, in part, by decreasing RPTC functions and inhibiting their repair.

Original languageEnglish (US)
Pages (from-to)F228-F236
JournalAmerican Journal of Physiology - Renal Physiology
Issue number2 45-2
StatePublished - Feb 1999
Externally publishedYes


  • Active sodium transport
  • Ascorbic acid
  • Cell repair
  • Cysteine conjugate
  • Epidermal growth factor
  • Mitochondrial function
  • Oxygen consumption
  • Regeneration
  • Renal proximal tubular cells
  • S-(1,2-dichlorovinyl)- L-cysteine
  • Sodium-coupled glucose uptake
  • Sodium-potassium adenosinetriphosphatase
  • Sublethal cell injury

ASJC Scopus subject areas

  • Physiology
  • Urology


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