Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae

Joe R. Delaney; Christopher Murakami; Annie Chou; Daniel Carr; Jennifer Schleit; George L. Sutphin; Elroy H. An; Anthony S. Castanza; Marissa Fletcher; Sarani Goswami; Sean Higgins; Mollie Holmberg; Jessica Hui; Monika Jelic; Ki Soo Jeong; Jin R. Kim; Shannon Klum; Eric Liao; Michael S. Lin; Winston Lo; Hillary Miller; Richard Moller; Zhao J. Peng; Tom Pollard; Prarthana Pradeep; Dillon Pruett; Dilreet Rai; Vanessa Ros; Alex Schuster; Minnie Singh; Benjamin L. Spector; Helen Vander Wende; Adrienne M. Wang; Brian M. Wasko; Brady Olsen; Matt Kaeberlein

doi:10.1016/j.exger.2012.12.001

Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae

Joe R. Delaney, Christopher Murakami, Annie Chou, Daniel Carr, Jennifer Schleit, George L. Sutphin, Elroy H. An, Anthony S. Castanza, Marissa Fletcher, Sarani Goswami, Sean Higgins, Mollie Holmberg, Jessica Hui, Monika Jelic, Ki Soo Jeong, Jin R. Kim, Shannon Klum, Eric Liao, Michael S. Lin, Winston LoHillary Miller, Richard Moller, Zhao J. Peng, Tom Pollard, Prarthana Pradeep, Dillon Pruett, Dilreet Rai, Vanessa Ros, Alex Schuster, Minnie Singh, Benjamin L. Spector, Helen Vander Wende, Adrienne M. Wang, Brian M. Wasko, Brady Olsen, Matt Kaeberlein

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23. days of chronological age. We further show that among the viable portion of the control population aged 26. days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process.

Original language	English (US)
Pages (from-to)	1006-1013
Number of pages	8
Journal	Experimental Gerontology
Volume	48
Issue number	10
DOIs	https://doi.org/10.1016/j.exger.2012.12.001
State	Published - Oct 2013
Externally published	Yes

Keywords

Caloric restriction
Calorie restriction
Chronological lifespan
Dietary restriction
Glucose
Mitochondria
Replicative lifespan

ASJC Scopus subject areas

Biochemistry
Aging
Molecular Biology
Genetics
Endocrinology
Cell Biology

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10.1016/j.exger.2012.12.001

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Delaney, J. R., Murakami, C., Chou, A., Carr, D., Schleit, J., Sutphin, G. L., An, E. H., Castanza, A. S., Fletcher, M., Goswami, S., Higgins, S., Holmberg, M., Hui, J., Jelic, M., Jeong, K. S., Kim, J. R., Klum, S., Liao, E., Lin, M. S., ... Kaeberlein, M. (2013). Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae. Experimental Gerontology, 48(10), 1006-1013. https://doi.org/10.1016/j.exger.2012.12.001

Delaney, JR, Murakami, C, Chou, A, Carr, D, Schleit, J, Sutphin, GL, An, EH, Castanza, AS, Fletcher, M, Goswami, S, Higgins, S, Holmberg, M, Hui, J, Jelic, M, Jeong, KS, Kim, JR, Klum, S, Liao, E, Lin, MS, Lo, W, Miller, H, Moller, R, Peng, ZJ, Pollard, T, Pradeep, P, Pruett, D, Rai, D, Ros, V, Schuster, A, Singh, M, Spector, BL, Wende, HV, Wang, AM, Wasko, BM, Olsen, B & Kaeberlein, M 2013, 'Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae', Experimental Gerontology, vol. 48, no. 10, pp. 1006-1013. https://doi.org/10.1016/j.exger.2012.12.001

@article{2a9cb5c4eeba4aefb1c6d6b4ecfb993c,

title = "Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae",

abstract = "Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23. days of chronological age. We further show that among the viable portion of the control population aged 26. days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process.",

keywords = "Caloric restriction, Calorie restriction, Chronological lifespan, Dietary restriction, Glucose, Mitochondria, Replicative lifespan",

author = "Delaney, {Joe R.} and Christopher Murakami and Annie Chou and Daniel Carr and Jennifer Schleit and Sutphin, {George L.} and An, {Elroy H.} and Castanza, {Anthony S.} and Marissa Fletcher and Sarani Goswami and Sean Higgins and Mollie Holmberg and Jessica Hui and Monika Jelic and Jeong, {Ki Soo} and Kim, {Jin R.} and Shannon Klum and Eric Liao and Lin, {Michael S.} and Winston Lo and Hillary Miller and Richard Moller and Peng, {Zhao J.} and Tom Pollard and Prarthana Pradeep and Dillon Pruett and Dilreet Rai and Vanessa Ros and Alex Schuster and Minnie Singh and Spector, {Benjamin L.} and Wende, {Helen Vander} and Wang, {Adrienne M.} and Wasko, {Brian M.} and Brady Olsen and Matt Kaeberlein",

note = "Funding Information: This work was supported by NIH grant R01AG039390 to MK. Additional support for flow cytometry was provided by the University of Washington Nathan Shock Center of Excellence in the Basic Biology of Aging ( NIH P30AG013280 ). JRD and GLS were supported by NIH Training Grant T32AG000057 . JS and BMW were supported by NIH Training Grant T32ES007032 .",

year = "2013",

month = oct,

doi = "10.1016/j.exger.2012.12.001",

language = "English (US)",

volume = "48",

pages = "1006--1013",

journal = "Experimental Gerontology",

issn = "0531-5565",

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number = "10",

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T1 - Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae

AU - Delaney, Joe R.

AU - Murakami, Christopher

AU - Chou, Annie

AU - Carr, Daniel

AU - Schleit, Jennifer

AU - Sutphin, George L.

AU - An, Elroy H.

AU - Castanza, Anthony S.

AU - Fletcher, Marissa

AU - Goswami, Sarani

AU - Higgins, Sean

AU - Holmberg, Mollie

AU - Hui, Jessica

AU - Jelic, Monika

AU - Jeong, Ki Soo

AU - Kim, Jin R.

AU - Klum, Shannon

AU - Liao, Eric

AU - Lin, Michael S.

AU - Lo, Winston

AU - Miller, Hillary

AU - Moller, Richard

AU - Peng, Zhao J.

AU - Pollard, Tom

AU - Pradeep, Prarthana

AU - Pruett, Dillon

AU - Rai, Dilreet

AU - Ros, Vanessa

AU - Schuster, Alex

AU - Singh, Minnie

AU - Spector, Benjamin L.

AU - Wende, Helen Vander

AU - Wang, Adrienne M.

AU - Wasko, Brian M.

AU - Olsen, Brady

AU - Kaeberlein, Matt

N1 - Funding Information: This work was supported by NIH grant R01AG039390 to MK. Additional support for flow cytometry was provided by the University of Washington Nathan Shock Center of Excellence in the Basic Biology of Aging ( NIH P30AG013280 ). JRD and GLS were supported by NIH Training Grant T32AG000057 . JS and BMW were supported by NIH Training Grant T32ES007032 .

PY - 2013/10

Y1 - 2013/10

N2 - Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23. days of chronological age. We further show that among the viable portion of the control population aged 26. days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process.

AB - Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23. days of chronological age. We further show that among the viable portion of the control population aged 26. days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process.

KW - Caloric restriction

KW - Calorie restriction

KW - Chronological lifespan

KW - Dietary restriction

KW - Glucose

KW - Mitochondria

KW - Replicative lifespan

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JO - Experimental Gerontology

JF - Experimental Gerontology

IS - 10

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Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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