Diaphragm single-fiber weakness and loss of myosin in congestive heart failure rats

Hieronymus W.H. Van Hees, Henricus F.M. Van Der Heijden, Coen A.C. Ottenheijm, Leo M.A. Heunks, Cindy J.C. Pigmans, Freek W.A. Verheugt, Rene M.H.J. Brouwer, P. N.Richard Dekhuijzen

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Diaphragm weakness commonly occurs in patients with congestive heart failure (CHF) and is an independent predictor of mortality. However, the pathophysiology of diaphragm weakness is poorly understood. We hypothesized that CHF induces diaphragm weakness at the single-fiber level by decreasing myosin content. In addition, we hypothesized that myofibrillar Ca2+ sensitivity is decreased and cross-bridge kinetics are slower in CHF diaphragm fibers. Finally, we hypothesized that loss of myosin in CHF diaphragm weakness is associated with increased proteolytic activities of caspase-3 and the proteasome. In skinned diaphragm single fibers of rats with CHF, induced by left coronary artery ligation, maximum force generation was reduced by ∼35% (P < 0.01) compared with sham-operated animals for slow, 2a, and 2x fibers. In these CHF diaphragm fibers, myosin heavy chain content per half-sarcomere was concomitantly decreased (P < 0.01). Ca2+ sensitivity of force generation and the rate constant of tension redevelopment were significantly reduced in CHF diaphragm fibers compared with sham-operated animals for all fiber types. The cleavage activity of the proteolytic enzyme caspase-3 and the proteasome were ∼30% (P < 0.05) and ∼60% (P < 0.05) higher, respectively, in diaphragm homogenates from CHF rats than from sham-operated rats. The present study demonstrates diaphragm weakness at the single-fiber level in a myocardial infarct model of CHF. The reduced maximal force generation can be explained by a loss of myosin content in all fiber types and is associated with activation of caspase-3 and the proteasome. Furthermore, CHF decreases myofibrillar Ca2+ sensitivity and slows cross-bridge cycling kinetics in diaphragm fibers.

Original languageEnglish (US)
Pages (from-to)H819-H828
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume293
Issue number1
DOIs
StatePublished - Jul 2007
Externally publishedYes

Keywords

  • Ca sensitivity
  • Caspase-3
  • Cross-bridge cycling kinetics
  • Proteasome
  • Skinned muscle single fibers

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Diaphragm single-fiber weakness and loss of myosin in congestive heart failure rats'. Together they form a unique fingerprint.

Cite this