TY - JOUR
T1 - Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens
AU - Marner, Erin S.
AU - Wolk, Donna M.
AU - Carr, Jeanne
AU - Hewitt, Carolyn
AU - Dominguez, Lorraine L.
AU - Kovacs, Thomas
AU - Johnson, Desiree R.
AU - Hayden, Randall T.
PY - 2011/4
Y1 - 2011/4
N2 - Rapid detection of vancomycin-resistant enterococci (VRE) carriers could be useful to health care facilities to minimize transmission. To that end, we compared the performance of the Cepheid GeneXpert vanA/. vanB assay with that of direct and broth-enriched culture methods for detection of VRE from perianal swabs. Enterococci were cultivated on Enterococcosel™ agar with 8 μg/mL vancomycin, Bile Esculin Azide Agar with 6 μg/mL vancomycin, and Bile Esculin Azide Enterococcosel Broth. Compared to the reference standard (combination of direct agar plating, broth-enriched culture, and clinical chart review), the sensitivity, specificity, positive predictive value, and negative predictive value of the vanA/. vanB assay were 96.4%, 93.0%, 92.0%, and 96.9%, respectively (n = 184). The 95% limit of detection was 100 colony-forming units (CFU)/mL for vanA and 114 CFU/mL for vanB. In summary, the GeneXpert vanA/. vanB assay is a rapid and accurate method to identify vanA/. vanB-colonized patients for VRE screening programs that use perianal swab specimens.
AB - Rapid detection of vancomycin-resistant enterococci (VRE) carriers could be useful to health care facilities to minimize transmission. To that end, we compared the performance of the Cepheid GeneXpert vanA/. vanB assay with that of direct and broth-enriched culture methods for detection of VRE from perianal swabs. Enterococci were cultivated on Enterococcosel™ agar with 8 μg/mL vancomycin, Bile Esculin Azide Agar with 6 μg/mL vancomycin, and Bile Esculin Azide Enterococcosel Broth. Compared to the reference standard (combination of direct agar plating, broth-enriched culture, and clinical chart review), the sensitivity, specificity, positive predictive value, and negative predictive value of the vanA/. vanB assay were 96.4%, 93.0%, 92.0%, and 96.9%, respectively (n = 184). The 95% limit of detection was 100 colony-forming units (CFU)/mL for vanA and 114 CFU/mL for vanB. In summary, the GeneXpert vanA/. vanB assay is a rapid and accurate method to identify vanA/. vanB-colonized patients for VRE screening programs that use perianal swab specimens.
KW - Culture
KW - GeneXpert
KW - Real-time PCR
KW - VRE
UR - https://www.scopus.com/pages/publications/79952407235
UR - https://www.scopus.com/pages/publications/79952407235#tab=citedBy
U2 - 10.1016/j.diagmicrobio.2010.11.005
DO - 10.1016/j.diagmicrobio.2010.11.005
M3 - Article
C2 - 21396533
AN - SCOPUS:79952407235
SN - 0732-8893
VL - 69
SP - 382
EP - 389
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -