Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens

Erin S. Marner; Donna M. Wolk; Jeanne Carr; Carolyn Hewitt; Lorraine L. Dominguez; Thomas Kovacs; Desiree R. Johnson; Randall T. Hayden

doi:10.1016/j.diagmicrobio.2010.11.005

Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens

Erin S. Marner
, Donna M. Wolk
, Jeanne Carr
, Carolyn Hewitt
, Lorraine L. Dominguez
, Thomas Kovacs
, Desiree R. Johnson
, Randall T. Hayden

Pathology and Lab Medicine

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Rapid detection of vancomycin-resistant enterococci (VRE) carriers could be useful to health care facilities to minimize transmission. To that end, we compared the performance of the Cepheid GeneXpert vanA/. vanB assay with that of direct and broth-enriched culture methods for detection of VRE from perianal swabs. Enterococci were cultivated on Enterococcosel™ agar with 8 μg/mL vancomycin, Bile Esculin Azide Agar with 6 μg/mL vancomycin, and Bile Esculin Azide Enterococcosel Broth. Compared to the reference standard (combination of direct agar plating, broth-enriched culture, and clinical chart review), the sensitivity, specificity, positive predictive value, and negative predictive value of the vanA/. vanB assay were 96.4%, 93.0%, 92.0%, and 96.9%, respectively (n = 184). The 95% limit of detection was 100 colony-forming units (CFU)/mL for vanA and 114 CFU/mL for vanB. In summary, the GeneXpert vanA/. vanB assay is a rapid and accurate method to identify vanA/. vanB-colonized patients for VRE screening programs that use perianal swab specimens.

Original language	English (US)
Pages (from-to)	382-389
Number of pages	8
Journal	Diagnostic Microbiology and Infectious Disease
Volume	69
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2010.11.005
State	Published - Apr 2011

Keywords

Culture
GeneXpert
Real-time PCR
VRE

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2010.11.005

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Marner, E. S., Wolk, D. M., Carr, J., Hewitt, C., Dominguez, L. L., Kovacs, T., Johnson, D. R., & Hayden, R. T. (2011). Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens. Diagnostic Microbiology and Infectious Disease, 69(4), 382-389. https://doi.org/10.1016/j.diagmicrobio.2010.11.005

Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens. / Marner, Erin S.; Wolk, Donna M.; Carr, Jeanne et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 69, No. 4, 04.2011, p. 382-389.

Research output: Contribution to journal › Article › peer-review

Marner, ES, Wolk, DM, Carr, J, Hewitt, C, Dominguez, LL, Kovacs, T, Johnson, DR & Hayden, RT 2011, 'Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens', Diagnostic Microbiology and Infectious Disease, vol. 69, no. 4, pp. 382-389. https://doi.org/10.1016/j.diagmicrobio.2010.11.005

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abstract = "Rapid detection of vancomycin-resistant enterococci (VRE) carriers could be useful to health care facilities to minimize transmission. To that end, we compared the performance of the Cepheid GeneXpert vanA/. vanB assay with that of direct and broth-enriched culture methods for detection of VRE from perianal swabs. Enterococci were cultivated on Enterococcosel{\texttrademark} agar with 8 μg/mL vancomycin, Bile Esculin Azide Agar with 6 μg/mL vancomycin, and Bile Esculin Azide Enterococcosel Broth. Compared to the reference standard (combination of direct agar plating, broth-enriched culture, and clinical chart review), the sensitivity, specificity, positive predictive value, and negative predictive value of the vanA/. vanB assay were 96.4\%, 93.0\%, 92.0\%, and 96.9\%, respectively (n = 184). The 95\% limit of detection was 100 colony-forming units (CFU)/mL for vanA and 114 CFU/mL for vanB. In summary, the GeneXpert vanA/. vanB assay is a rapid and accurate method to identify vanA/. vanB-colonized patients for VRE screening programs that use perianal swab specimens.",

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Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens

Abstract

Keywords

ASJC Scopus subject areas

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