Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Chadi A. Hage; Thomas E. Davis; Lindsey Egan; Michelle Parker; Deanna Fuller; Ann M. LeMonte; Michelle Durkin; Patricia Connelly; L. Joseph Wheat; Deborah Blue-Hnidy; Kenneth S. Knox

doi:10.1016/j.rmed.2006.04.017

Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Chadi A. Hage
, Thomas E. Davis
, Lindsey Egan
, Michelle Parker
, Deanna Fuller
, Ann M. LeMonte
, Michelle Durkin
, Patricia Connelly
, L. Joseph Wheat
, Deborah Blue-Hnidy
, Kenneth S. Knox

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

Original language	English (US)
Pages (from-to)	43-47
Number of pages	5
Journal	Respiratory Medicine
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/j.rmed.2006.04.017
State	Published - Jan 2007
Externally published	Yes

Keywords

Blastomycosis
Bronchoalveolar lavage
Histoplasma antigen
Histoplasmosis
Second-generation assay

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1016/j.rmed.2006.04.017

Cite this

Hage, C. A., Davis, T. E., Egan, L., Parker, M., Fuller, D., LeMonte, A. M., Durkin, M., Connelly, P., Joseph Wheat, L., Blue-Hnidy, D., & Knox, K. S. (2007). Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. Respiratory Medicine, 101(1), 43-47. https://doi.org/10.1016/j.rmed.2006.04.017

Hage, CA, Davis, TE, Egan, L, Parker, M, Fuller, D, LeMonte, AM, Durkin, M, Connelly, P, Joseph Wheat, L, Blue-Hnidy, D & Knox, KS 2007, 'Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay', Respiratory Medicine, vol. 101, no. 1, pp. 43-47. https://doi.org/10.1016/j.rmed.2006.04.017

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title = "Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay",

abstract = "Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84\%) cases of histoplasmosis and 5 of 6 (83.3\%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68\%) and 4 of 6 (66.7\%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0\%). Specificity was 98.2\% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.",

keywords = "Blastomycosis, Bronchoalveolar lavage, Histoplasma antigen, Histoplasmosis, Second-generation assay",

author = "Hage, \{Chadi A.\} and Davis, \{Thomas E.\} and Lindsey Egan and Michelle Parker and Deanna Fuller and LeMonte, \{Ann M.\} and Michelle Durkin and Patricia Connelly and \{Joseph Wheat\}, L. and Deborah Blue-Hnidy and Knox, \{Kenneth S.\}",

year = "2007",

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doi = "10.1016/j.rmed.2006.04.017",

language = "English (US)",

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AU - Hage, Chadi A.

AU - Davis, Thomas E.

AU - Egan, Lindsey

AU - Parker, Michelle

AU - Fuller, Deanna

AU - LeMonte, Ann M.

AU - Durkin, Michelle

AU - Connelly, Patricia

AU - Joseph Wheat, L.

AU - Blue-Hnidy, Deborah

AU - Knox, Kenneth S.

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N2 - Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

AB - Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

KW - Blastomycosis

KW - Bronchoalveolar lavage

KW - Histoplasma antigen

KW - Histoplasmosis

KW - Second-generation assay

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M3 - Article

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SN - 0954-6111

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IS - 1

ER -

Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Abstract

Keywords

ASJC Scopus subject areas

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