Developmental profiling of microRNAs in the human embryonic inner ear

Duncan M. Chadly, Jennifer Best, Cong Ran, Małgorzata Bruska, Witold Woźniak, Bartosz Kempisty, Mark Schwartz, Bonnie LaFleur, B. J. Kerns, John A. Kessler, Akihiro J. Matsuoka

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Due to the extreme inaccessibility of fetal human inner ear tissue, defining of the microRNAs (miRNAs) that regulate development of the inner ear has relied on animal tissue. In the present study, we performed the first miRNA sequencing of otic precursors in human specimens. Using HTG miRNA Whole Transcriptome assays, we examined miRNA expression in the cochleovestibular ganglion (CVG), neural crest (NC), and otic vesicle (OV) from paraffin embedded (FFPE) human specimens in the Carnegie developmental stages 13–15. We found that in human embryonic tissues, there are different patterns of miRNA expression in the CVG, NC and OV. In particular, members of the miR-183 family (miR-96, miR-182, and miR-183) are differentially expressed in the CVG compared to NC and OV at Carnegie developmental stage 13. We further identified transcription factors that are differentially targeted in the CVG compared to the other tissues from stages 13–15, and we performed gene set enrichment analyses to determine differentially regulated pathways that are relevant to CVG development in humans. These findings not only provide insight into the mechanisms governing the development of the human inner ear, but also identify potential signaling pathways for promoting regeneration of the spiral ganglion and other components of the inner ear.

Original languageEnglish (US)
Article numbere0191452
JournalPloS one
Issue number1
StatePublished - Jan 2018
Externally publishedYes

ASJC Scopus subject areas

  • General


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