TY - JOUR
T1 - Development of transgenic cabbage (Brassica oleracea var. capitata) for insect resistance by agrobacterium tumefaciens-mediated transformation
AU - Jin, R. G.
AU - Liu, Y. B.
AU - Tabashnik, B. E.
AU - Borthakur, D.
N1 - Funding Information:
This research was supported by USDA-CSRS TSTAR grant 95-34135-1766. We wish to thank Mike Koziel, CIBA Geigy (now Novartis Seeds) and Byung Shin, Genetic Engineering Research Institute, Taejon, South Korea for providing the synthetic cry1Ab3 and cry1Ia3 genes, respectively. Agrobacterium tumefaciens EHA105 was kindly provided by Stanton Gelvin. We also thank David Webb for helping us with the figures. Paper No. 4469 of the
PY - 2000
Y1 - 2000
N2 - Transgenic head cabbage (Brassica oleracea var. capitata), resistant to diamondback moth (Plutella xylostella) larvae, was developed through Agrobacterium tumefaciens-mediated transformation with Bacillus thuringiensis (Bt) cry genes using a modified procedure. Factors important for transformation included cabbage cultivar; preculture and coculture of explants on a callus initiation medium; use of appropriate amount; and delay in initial application of selective agents. A total of 15 independent transformed lines with over 100 plants were obtained from several transformation experiments, representing an overall transformation efficiency of ~ 1%. Cabbage plants transformed with a synthetic Bt gene, cry1Ab3, were all resistant to larvae of the diamondback moth, whereas all plants transgenic for cry1Ia3, a wild-type Bt gene, were susceptible. As a first step towards testing the hypothesis that reduced exposure of Bt to target insects would delay the evolution of insect resistance to Bt, cry1Ab3 expression was put under the transcriptional control of the soybean wound-inducible vspB promoter and transgenic cabbage was obtained. Insect bioassay showed that such plants were all resistant to diamondback moth even without induction for the expression of Bt.
AB - Transgenic head cabbage (Brassica oleracea var. capitata), resistant to diamondback moth (Plutella xylostella) larvae, was developed through Agrobacterium tumefaciens-mediated transformation with Bacillus thuringiensis (Bt) cry genes using a modified procedure. Factors important for transformation included cabbage cultivar; preculture and coculture of explants on a callus initiation medium; use of appropriate amount; and delay in initial application of selective agents. A total of 15 independent transformed lines with over 100 plants were obtained from several transformation experiments, representing an overall transformation efficiency of ~ 1%. Cabbage plants transformed with a synthetic Bt gene, cry1Ab3, were all resistant to larvae of the diamondback moth, whereas all plants transgenic for cry1Ia3, a wild-type Bt gene, were susceptible. As a first step towards testing the hypothesis that reduced exposure of Bt to target insects would delay the evolution of insect resistance to Bt, cry1Ab3 expression was put under the transcriptional control of the soybean wound-inducible vspB promoter and transgenic cabbage was obtained. Insect bioassay showed that such plants were all resistant to diamondback moth even without induction for the expression of Bt.
KW - Brassica
KW - Diamondback moth
KW - Genetic transformation
KW - Inducible expression
KW - cry genes
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U2 - 10.1007/s11627-000-0043-1
DO - 10.1007/s11627-000-0043-1
M3 - Article
AN - SCOPUS:0033796874
SN - 1054-5476
VL - 36
SP - 231
EP - 237
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
IS - 4
ER -