Development of monoclonal antibodies for detection of necrotizing hepatopancreatitis in penaeid shrimp

Deborah J. Bradley-Dunlop, Carlos Pantoja, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    19 Scopus citations

    Abstract

    Monoclonal antibodies (MAbs) were produced against necrotizing hepatopancreatitis bacteria (NHP-B) of penaeid shrimp. The MAbs tested in dot-immunoblot (D-IB) assays were capable of detecting the NHP-B in hepatopancreas samples collected from moribund juvenile Litopenaeus vannamei during an experimentally induced NHP-B infection. The MAbs were also screened by immunohistochemistry (IHC) using case submissions that were determined to be infected not only by histology, but also polymerase chain reaction (PCR) and in situ hybridization (ISH) assays using specific digoxigenin (DIG)-labeled probes on histological sections prepared from naturally infected shrimp. Two of the MAbs were chosen for development of detection methods for NHP. The MAbs were tested using IHC methods on Davidson's alcohol-formalin-acetic acid (AFA) fixed tissue sections and identified NHP-B infected cells and tissues in a pattern similar to that seen with DIG-labeled NHP-specific gene probes. None of the MAbs reacted with tissue from specific pathogen-free (SPF) shrimp or with shrimp tissues infected with a rickettsia-like bacteria, Vibrio sp., Campylobacter sp., and Spiroplasma sp. The MAbs were found to be negative against these other organisms, demonstrating that they are species specific and useful for rapid diagnostic detection of NHP-B.

    Original languageEnglish (US)
    Pages (from-to)233-240
    Number of pages8
    JournalDiseases of aquatic organisms
    Volume60
    Issue number3
    DOIs
    StatePublished - Sep 8 2004

    Keywords

    • Dot-immunoblot
    • Immunohistochemistry
    • Monoclonal antibodies
    • NHP
    • Necrotizing hepatopanceatitis

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Aquatic Science

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