Abstract
An analytical procedure for the analysis of 9-(β-D-arabinofuranosyl)-adenine (ara-A) was developed and used to determine its solubility as well as stability. A reversed-phase high performance liquid chromatographic method was used to quantitate intact drug and to determine its degradation products in aqueous solutions. The solubility of ara-A was determined in different cosolvent-water mixtures as well as in different pH-buffers. The aqueous solubility of ara-A increased exponentially with a linear increase in the volume fraction of the cosolvent. Stability studies indicate that ara-A is relatively stable in dimethylsulfoxide, dimethylacetamide, and dimethylformamide. In vitro precipitation was studied to evaluate the relative potential for precipitation of the drug on injection. Based on these findings, ara-A may be dissolved in the 70% dimethylsulfoxide-water mixture at 15 mg/ml and injected at a rate of 2 ml/min.
Original language | English (US) |
---|---|
Pages (from-to) | 15-20 |
Number of pages | 6 |
Journal | Journal of Parenteral Science and Technology |
Volume | 41 |
Issue number | 1 |
State | Published - 1987 |
ASJC Scopus subject areas
- Pharmaceutical Science