Development of a specific pathogen free population of the Chinese fleshy prawn, Fenneropenaeus chinensis: Part II. Secondary quarantine

Oscar L. Hennig, Steve M. Arce, Shaun M. Moss, Carlos R. Pantoja, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    5 Scopus citations


    The Oceanic Institute (OI), in collaboration with researchers from the University of Arizona (UAZ), established a population of specific pathogen free (SPF) Fenneropenaeus chinensis through Secondary Quarantine (SQ) in Kona, Hawaii. Shrimp were 0.38 g (S.D. ± 0.13 g) when they entered the nursery/growout module of the SQ facility. Juveniles from five maternal families were stocked in separate 230-L nursery tanks, as was a sixth family comprised of shrimp from two of the seven original spawns. After tagging, representative shrimp from all six families were stocked in a 50-m2 concrete raceway that was enclosed in a biosecure greenhouse. After 155 days in the growout module, mean shrimp weight was 23.4 g (S.D. ± 7.25 g) and females (29.1 ± 4.61 g) were 74% larger than males (16.7 ± 2.44 g). A Sprung Structure™ was erected to enclose the biosecure maturation and hatchery module of the SQ facility. Broodstock were subjected to an acclimation process where they were kept in seawater at 22°C, followed by a reduction in water temperature of 1°C/day until the temperature reached 17°C. At this time, unilateral eyestalk ablation was performed on female broodstock and water temperature was further reduced at the same rate until it reached 12°C. After 2 weeks, water temperature was raised to 17°C at a rate of 1°C/day to induce ovarian development. Production of an F1 generation of Fe. chinensis was successfully accomplished using artificial insemination, and representative shrimp from this generation have tested negative for all specifically listed pathogens using histology and PCR/RT-PCR. Of particular interest with Fe. chinensis is their cold tolerance. This species may represent a valuable resource for shrimp farmers in temperate and sub-tropical climates to grow a second "winter" crop, thereby increasing production and profitability for the shrimp farmer.

    Original languageEnglish (US)
    Pages (from-to)579-585
    Number of pages7
    Issue number3-4
    StatePublished - Dec 30 2005


    • Biosecure quarantine
    • Cold tolerance
    • Fenneropenaeus chinensis
    • SPF

    ASJC Scopus subject areas

    • Aquatic Science


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