Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo

Anita Mahadevan-Jansen, Michele Follen Mitchell, Nirmala Ramanujam, Urs Utzinger, Rebecca Richards-Kortum

Research output: Contribution to journalArticlepeer-review

197 Scopus citations


The goal of this study was to develop a compact fiber optic probe to measure near infrared Raman spectra of human cervical tissue in vivo for the clinical diagnosis of cervical precancers. A Raman spectrometer and fiber optic probe were designed, constructed and tested. The probe was first tested using standards with known Raman spectra, and then the probe was used to acquire Raman spectra from normal and precancerous cervical tissue in vivo. Raman spectra of cervical tissue could be acquired in vivo in 90 s using incident powers comparable to the threshold limit values for laser exposure of the skin. Although some silica signal obscured tissue Raman bands below 900 cm-1, Raman features from cervical tissue could clearly be discerned with an acceptable signal-to-noise ratio above 900 cm-1. The success of the Raman probe described here indicates that near infrared Raman spectra can be measured in vivo from cervical tissues. Increasing the power of the excitation source could reduce the integration time to below 20 s.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalPhotochemistry and Photobiology
Issue number3
StatePublished - Sep 1998

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry


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