Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped βTC3 cell cultures

K. K. Papas; R. C. Long; A. Sambanis; I. Constantinidis

doi:10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U

Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped βTC3 cell cultures

K. K. Papas, R. C. Long, A. Sambanis, I. Constantinidis

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

The effects of 3- and 4-day-long hypoxic episodes on the metabolic and secretory activities and on the levels of intracellular metabolites detectable by phosphorus-31 nuclear magnetic resonance of alginate/poly-L-lysine/alginate entrapped βCT3 mouse insulinomas continuously perfused with culture medium were studied. Results show that upon decreasing the oxygen concentration in the surrounding medium, the encapsulated cell system reached a new, lower metabolic and secretory state. Upon restoration of the oxygen concentration in the perfusion medium, all parameters returned to their prehypoxic levels within 2 to 3 days.

Original language	English (US)
Pages (from-to)	231-237
Number of pages	7
Journal	Biotechnology and Bioengineering
Volume	66
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U
State	Published - 1999
Externally published	Yes

Keywords

Bioartificial pancreas
Bioenergetics
Cell encapsulation
Insulin secretion
Nuclear magnetic resonance (NMR) spectroscopy
Oxygen effects
βTC3 cells

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U

Cite this

@article{371ad14d9163436f869d2bf85170c190,

title = "Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped βTC3 cell cultures",

abstract = "The effects of 3- and 4-day-long hypoxic episodes on the metabolic and secretory activities and on the levels of intracellular metabolites detectable by phosphorus-31 nuclear magnetic resonance of alginate/poly-L-lysine/alginate entrapped βCT3 mouse insulinomas continuously perfused with culture medium were studied. Results show that upon decreasing the oxygen concentration in the surrounding medium, the encapsulated cell system reached a new, lower metabolic and secretory state. Upon restoration of the oxygen concentration in the perfusion medium, all parameters returned to their prehypoxic levels within 2 to 3 days.",

keywords = "Bioartificial pancreas, Bioenergetics, Cell encapsulation, Insulin secretion, Nuclear magnetic resonance (NMR) spectroscopy, Oxygen effects, βTC3 cells",

author = "Papas, {K. K.} and Long, {R. C.} and A. Sambanis and I. Constantinidis",

year = "1999",

doi = "10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U",

language = "English (US)",

volume = "66",

pages = "231--237",

journal = "Biotechnology and Bioengineering",

issn = "0006-3592",

publisher = "Wiley-VCH Verlag",

number = "4",

}

TY - JOUR

T1 - Development of a bioartificial pancreas

T2 - II. Effects of oxygen on long- term entrapped βTC3 cell cultures

AU - Papas, K. K.

AU - Long, R. C.

AU - Sambanis, A.

AU - Constantinidis, I.

PY - 1999

Y1 - 1999

N2 - The effects of 3- and 4-day-long hypoxic episodes on the metabolic and secretory activities and on the levels of intracellular metabolites detectable by phosphorus-31 nuclear magnetic resonance of alginate/poly-L-lysine/alginate entrapped βCT3 mouse insulinomas continuously perfused with culture medium were studied. Results show that upon decreasing the oxygen concentration in the surrounding medium, the encapsulated cell system reached a new, lower metabolic and secretory state. Upon restoration of the oxygen concentration in the perfusion medium, all parameters returned to their prehypoxic levels within 2 to 3 days.

AB - The effects of 3- and 4-day-long hypoxic episodes on the metabolic and secretory activities and on the levels of intracellular metabolites detectable by phosphorus-31 nuclear magnetic resonance of alginate/poly-L-lysine/alginate entrapped βCT3 mouse insulinomas continuously perfused with culture medium were studied. Results show that upon decreasing the oxygen concentration in the surrounding medium, the encapsulated cell system reached a new, lower metabolic and secretory state. Upon restoration of the oxygen concentration in the perfusion medium, all parameters returned to their prehypoxic levels within 2 to 3 days.

KW - Bioartificial pancreas

KW - Bioenergetics

KW - Cell encapsulation

KW - Insulin secretion

KW - Nuclear magnetic resonance (NMR) spectroscopy

KW - Oxygen effects

KW - βTC3 cells

UR - http://www.scopus.com/inward/record.url?scp=0033376083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033376083&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U

DO - 10.1002/(SICI)1097-0290(1999)66:4<231::AID-BIT4>3.0.CO;2-U

M3 - Article

C2 - 10578093

AN - SCOPUS:0033376083

SN - 0006-3592

VL - 66

SP - 231

EP - 237

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 4

ER -

Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped βTC3 cell cultures

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this