Development and testing of a high-density cDNA microarray resource for cattle

Steven P. Suchyta; Sue Sipkovsky; Rachael Kruska; Abra Jeffers; Amanda McNulty; Matthew J. Coussens; Robert J. Tempelman; Robert G. Halgren; Peter M. Saama; Dale E. Bauman; Yves R. Boisclair; Jeanne L. Burton; Robert J. Collier; Edward J. DePeters; Theodore A. Ferris; Matthew C. Lucy; Mark A. McGuire; Juan F. Medrano; Thomas R. Overton; Timothy P. Smith; George W. Smith; Tad S. Sonstegard; James N. Spain; Donald E. Spiers; Jianbo Yao; Paul M. Coussens

doi:10.1152/physiolgenomics.00094.2003

Development and testing of a high-density cDNA microarray resource for cattle

Steven P. Suchyta, Sue Sipkovsky, Rachael Kruska, Abra Jeffers, Amanda McNulty, Matthew J. Coussens, Robert J. Tempelman, Robert G. Halgren, Peter M. Saama, Dale E. Bauman, Yves R. Boisclair, Jeanne L. Burton, Robert J. Collier, Edward J. DePeters, Theodore A. Ferris, Matthew C. Lucy, Mark A. McGuire, Juan F. Medrano, Thomas R. Overton, Timothy P. SmithGeorge W. Smith, Tad S. Sonstegard, James N. Spain, Donald E. Spiers, Jianbo Yao, Paul M. Coussens

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortium's (NBFGC) expressed sequence tag (EST) collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues. The NBFGC microarray has been tested for false-positive rates using self-self hybridizations and was shown to yield robust results in test microarray experiments. A web-accessible database has been established to provide pertinent data related to NBFGC clones, including sequence data, BLAST results, and ontology information. The NBFGC microarray represents the largest cDNA microarray for a livestock species prepared to date and should prove to be a valuable tool in studying genome-wide gene expression in cattle.

Original language	English (US)
Pages (from-to)	158-164
Number of pages	7
Journal	Physiological Genomics
Volume	15
DOIs	https://doi.org/10.1152/physiolgenomics.00094.2003
State	Published - Jan 2004

Keywords

Bovine
Expressed sequence tag

ASJC Scopus subject areas

Physiology
Genetics

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10.1152/physiolgenomics.00094.2003

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Suchyta, S. P., Sipkovsky, S., Kruska, R., Jeffers, A., McNulty, A., Coussens, M. J., Tempelman, R. J., Halgren, R. G., Saama, P. M., Bauman, D. E., Boisclair, Y. R., Burton, J. L., Collier, R. J., DePeters, E. J., Ferris, T. A., Lucy, M. C., McGuire, M. A., Medrano, J. F., Overton, T. R., ... Coussens, P. M. (2004). Development and testing of a high-density cDNA microarray resource for cattle. Physiological Genomics, 15, 158-164. https://doi.org/10.1152/physiolgenomics.00094.2003

Suchyta, SP, Sipkovsky, S, Kruska, R, Jeffers, A, McNulty, A, Coussens, MJ, Tempelman, RJ, Halgren, RG, Saama, PM, Bauman, DE, Boisclair, YR, Burton, JL, Collier, RJ, DePeters, EJ, Ferris, TA, Lucy, MC, McGuire, MA, Medrano, JF, Overton, TR, Smith, TP, Smith, GW, Sonstegard, TS, Spain, JN, Spiers, DE, Yao, J & Coussens, PM 2004, 'Development and testing of a high-density cDNA microarray resource for cattle', Physiological Genomics, vol. 15, pp. 158-164. https://doi.org/10.1152/physiolgenomics.00094.2003

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AU - Smith, George W.

AU - Sonstegard, Tad S.

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AU - Coussens, Paul M.

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Development and testing of a high-density cDNA microarray resource for cattle

Abstract

Keywords

ASJC Scopus subject areas

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