Development and Application of Multidimensional Lipid Libraries to Investigate Lipidomic Dysregulation Related to Smoke Inhalation Injury Severity

Kaylie I. Kirkwood, Michael W. Christopher, Jefferey L. Burgess, Sally R. Littau, Kevin Foster, Karen Richey, Brian S. Pratt, Nicholas Shulman, Kaipo Tamura, Michael J. MacCoss, Brendan X. MacLean, Erin S. Baker

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The implication of lipid dysregulation in diseases, toxic exposure outcomes, and inflammation has brought great interest to lipidomic studies. However, lipids have proven to be analytically challenging due to their highly isomeric nature and vast concentration ranges in biological matrices. Therefore, multidimensional techniques such as those integrating liquid chromatography, ion mobility spectrometry, collision-induced dissociation, and mass spectrometry (LC-IMS-CID-MS) have been implemented to separate lipid isomers as well as provide structural information and increased identification confidence. These data sets are however extremely large and complex, resulting in challenges for data processing and annotation. Here, we have overcome these challenges by developing sample-specific multidimensional lipid libraries using the freely available software Skyline. Specifically, the human plasma library developed for this work contains over 500 unique lipids and is combined with adapted Skyline functions such as indexed retention time (iRT) for retention time prediction and IMS drift time filtering for enhanced selectivity. For comparison with other studies, this database was used to annotate LC-IMS-CID-MS data from a NIST SRM 1950 extract. The same workflow was then utilized to assess plasma and bronchoalveolar lavage fluid (BALF) samples from patients with varying degrees of smoke inhalation injury to identify lipid-based patient prognostic and diagnostic markers.

Original languageEnglish (US)
Pages (from-to)232-242
Number of pages11
JournalJournal of Proteome Research
Volume21
Issue number1
DOIs
StatePublished - Jan 7 2022

Keywords

  • data annotation
  • ion mobility spectrometry
  • lipidomics
  • smoke inhalation
  • spectral libraries

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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