Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector

D. R. Heine; M. B. Denton; T. D. Schlabach

doi:10.1021/ac00238a025

Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector

D. R. Heine, M. B. Denton, T. D. Schlabach

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25 Scopus citations

Abstract

Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na₂HPO₄(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.

Original language	English (US)
Pages (from-to)	81-84
Number of pages	4
Journal	Analytical Chemistry
Volume	54
Issue number	1
DOIs	https://doi.org/10.1021/ac00238a025
State	Published - Jan 1982
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac00238a025

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title = "Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector",

abstract = "Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.",

author = "Heine, {D. R.} and Denton, {M. B.} and Schlabach, {T. D.}",

year = "1982",

month = jan,

doi = "10.1021/ac00238a025",

language = "English (US)",

volume = "54",

pages = "81--84",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector

AU - Heine, D. R.

AU - Denton, M. B.

AU - Schlabach, T. D.

PY - 1982/1

Y1 - 1982/1

N2 - Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.

AB - Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 1

ER -

Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector

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