Determination of melanotan II in rabbit urine using solid-phase extraction sample preparation followed by reversed-phase high-performance liquid chromatography

Andrea E. Walters; Paul B. Myrdal; Sirirat Pinsuwan; Aldona M. Manka; Samuel H. Yalkowsky

doi:10.1016/S0378-4347(96)00389-1

Determination of melanotan II in rabbit urine using solid-phase extraction sample preparation followed by reversed-phase high-performance liquid chromatography

Andrea E. Walters, Paul B. Myrdal, Sirirat Pinsuwan, Aldona M. Manka, Samuel H. Yalkowsky

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6 Scopus citations

Abstract

A solid-phase extraction (SPE) method has been developed for the isolation of melanotan II from rabbit urine. The proposed extraction method makes it possible to selectively isolate melanotan II without significant loss of the peptide. Standard curves obtained from high-performance liquid chromatographic (HPLC) analysis of spiked urine extracts are linear from 0.1 to 4.0 μg/ml. The analytical method is shown to be highly reproducible, giving a relative standard deviation of less than 5% for both between-day and same-day analyses. The accuracy of the method obtained from standard plots ranges from -3.3 to 3.1%.

Original language	English (US)
Pages (from-to)	99-103
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Applications
Volume	690
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(96)00389-1
State	Published - Mar 7 1997

Keywords

Melanotan II

ASJC Scopus subject areas

General Chemistry

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10.1016/S0378-4347(96)00389-1

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Determination of melanotan II in rabbit urine using solid-phase extraction sample preparation followed by reversed-phase high-performance liquid chromatography. / Walters, Andrea E.; Myrdal, Paul B.; Pinsuwan, Sirirat et al.
In: Journal of Chromatography B: Biomedical Applications, Vol. 690, No. 1-2, 07.03.1997, p. 99-103.

Research output: Contribution to journal › Article › peer-review

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abstract = "A solid-phase extraction (SPE) method has been developed for the isolation of melanotan II from rabbit urine. The proposed extraction method makes it possible to selectively isolate melanotan II without significant loss of the peptide. Standard curves obtained from high-performance liquid chromatographic (HPLC) analysis of spiked urine extracts are linear from 0.1 to 4.0 μg/ml. The analytical method is shown to be highly reproducible, giving a relative standard deviation of less than 5% for both between-day and same-day analyses. The accuracy of the method obtained from standard plots ranges from -3.3 to 3.1%.",

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AU - Walters, Andrea E.

AU - Myrdal, Paul B.

AU - Pinsuwan, Sirirat

AU - Manka, Aldona M.

AU - Yalkowsky, Samuel H.

PY - 1997/3/7

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N2 - A solid-phase extraction (SPE) method has been developed for the isolation of melanotan II from rabbit urine. The proposed extraction method makes it possible to selectively isolate melanotan II without significant loss of the peptide. Standard curves obtained from high-performance liquid chromatographic (HPLC) analysis of spiked urine extracts are linear from 0.1 to 4.0 μg/ml. The analytical method is shown to be highly reproducible, giving a relative standard deviation of less than 5% for both between-day and same-day analyses. The accuracy of the method obtained from standard plots ranges from -3.3 to 3.1%.

AB - A solid-phase extraction (SPE) method has been developed for the isolation of melanotan II from rabbit urine. The proposed extraction method makes it possible to selectively isolate melanotan II without significant loss of the peptide. Standard curves obtained from high-performance liquid chromatographic (HPLC) analysis of spiked urine extracts are linear from 0.1 to 4.0 μg/ml. The analytical method is shown to be highly reproducible, giving a relative standard deviation of less than 5% for both between-day and same-day analyses. The accuracy of the method obtained from standard plots ranges from -3.3 to 3.1%.

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Determination of melanotan II in rabbit urine using solid-phase extraction sample preparation followed by reversed-phase high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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