Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry

Jessica A. Miller; Iman A. Hakim; Cynthia Thomson; Patricia Thompson; Hsiao-Hui Chow

doi:10.1016/j.jchromb.2008.06.002

Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry

Jessica A. Miller, Iman A. Hakim, Cynthia Thomson, Patricia Thompson, Hsiao-Hui Chow

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1 g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.

Original language	English (US)
Pages (from-to)	68-73
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	870
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2008.06.002
State	Published - Jul 1 2008

Keywords

Adipose
GC-MS
d-Limonene

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2008.06.002

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title = "Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry",

abstract = "We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1 g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.",

keywords = "Adipose, GC-MS, d-Limonene",

author = "Miller, {Jessica A.} and Hakim, {Iman A.} and Cynthia Thomson and Patricia Thompson and Hsiao-Hui Chow",

note = "Funding Information: This work was partially supported by the Arizona Cancer Center Core Grant (P30-CA-23074) and DOD Idea Award (BC061529).",

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doi = "10.1016/j.jchromb.2008.06.002",

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T1 - Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry

AU - Miller, Jessica A.

AU - Hakim, Iman A.

AU - Thomson, Cynthia

AU - Thompson, Patricia

AU - Chow, Hsiao-Hui

N1 - Funding Information: This work was partially supported by the Arizona Cancer Center Core Grant (P30-CA-23074) and DOD Idea Award (BC061529).

PY - 2008/7/1

Y1 - 2008/7/1

N2 - We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1 g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.

AB - We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1 g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.

KW - Adipose

KW - GC-MS

KW - d-Limonene

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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