Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent

Sanhita Sinharay; Gabriela Fernández-Cuervo; Jasmine P. Acfalle; Mark D. Pagel

doi:10.1002/chem.201600685

Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent

Sanhita Sinharay, Gabriela Fernández-Cuervo, Jasmine P. Acfalle, Mark D. Pagel

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.

Original language	English (US)
Pages (from-to)	6491-6495
Number of pages	5
Journal	Chemistry - A European Journal
Volume	22
Issue number	19
DOIs	https://doi.org/10.1002/chem.201600685
State	Published - May 4 2016

Keywords

NMR imaging
diagnostic agents
enzyme kinetics
kinetics
sulfatases

ASJC Scopus subject areas

General Chemistry
Catalysis
Organic Chemistry

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10.1002/chem.201600685

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title = "Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent",

abstract = "A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.",

keywords = "NMR imaging, diagnostic agents, enzyme kinetics, kinetics, sulfatases",

author = "Sanhita Sinharay and Gabriela Fern{\'a}ndez-Cuervo and Acfalle, \{Jasmine P.\} and Pagel, \{Mark D.\}",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH\&Co. KGaA, Weinheim.",

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AU - Fernández-Cuervo, Gabriela

AU - Acfalle, Jasmine P.

AU - Pagel, Mark D.

PY - 2016/5/4

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AB - A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.

KW - NMR imaging

KW - diagnostic agents

KW - enzyme kinetics

KW - kinetics

KW - sulfatases

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Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent

Abstract

Keywords

ASJC Scopus subject areas

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