Detection of infectious myonecrosis virus (IMNV) of penaeid shrimp by reverse-transcriptase polymerase chain reaction (RT-PCR)

Bonnie T. Poulos, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    43 Scopus citations

    Abstract

    Infectious myonecrosis virus (IMNV) infecting cultured Litopenaeus vannamei in Brazil is & double-stranded RNA virus that causes a slowly progressive disease with cumulative mortalities of up to 70%. The disease is currently diagnosed using a combination of gross signs (primarily skeletal tail muscle necrosis with white opaque discoloration), histopathology, and in situ hybridization with a digoxigenin-labeled gene probe. A rapid and sensitive method for definitive diagnosis of the disease was developed using reverse-transcriptase polymerase chain reaction (RT-PCR). Two primer sets were used to detect 328 and 139 bp amplicons in a nested RT-PCR assay. Using RNA extracted from purified virions, the first step reaction detected 100 copies of the IMNV viral genome whereas the nested step detected 10 copies. The primers were shown to be specific for IMNV and no amplicons were detected using RNA extracted from shrimp infected with other penaeid shrimp viruses (Taura syndrome virus [TSV], yellowhead virus [YHV], infectious hypodermal hematopoietic necrosis virus [IHHNV] and white spot syndrome virus [WSSV]).

    Original languageEnglish (US)
    Pages (from-to)69-72
    Number of pages4
    JournalDiseases of aquatic organisms
    Volume73
    Issue number1
    DOIs
    StatePublished - Nov 21 2006

    Keywords

    • IMNV
    • Infectious myonecrosis
    • Penaeid shrimp
    • RT-PCR

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Aquatic Science

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