Abstract
A procedure for the rapid identification of glutathione S-transferase isozymes from rat liver in polyacrylamide gels is described. The isozymes are separated by electrofocusing and then identified by bathing the gels in a solution containing substrates and scanning the gels at the appropriate wavelength for the appearance of product. Increase in absorbance as a function of time delineates areas containing enzyme from artifacts within the gel. This technique should be useful for the identification of isozymes of glutathione S-transferase in other tissues and also other species. Also, the technique provides for rapid confirmation of homogeneity of the isozymes of glutathione S-transferase.
Original language | English (US) |
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Pages (from-to) | 344-348 |
Number of pages | 5 |
Journal | Analytical Biochemistry |
Volume | 116 |
Issue number | 2 |
DOIs | |
State | Published - Sep 15 1981 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology