A procedure for the rapid identification of glutathione S-transferase isozymes from rat liver in polyacrylamide gels is described. The isozymes are separated by electrofocusing and then identified by bathing the gels in a solution containing substrates and scanning the gels at the appropriate wavelength for the appearance of product. Increase in absorbance as a function of time delineates areas containing enzyme from artifacts within the gel. This technique should be useful for the identification of isozymes of glutathione S-transferase in other tissues and also other species. Also, the technique provides for rapid confirmation of homogeneity of the isozymes of glutathione S-transferase.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Sep 15 1981|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology