Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

Ronald R. Watson; David Solkoff; James Y. Wang; Keifong Seeto

doi:10.1016/S0741-8329(98)00014-7

Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

Ronald R. Watson, David Solkoff, James Y. Wang, Keifong Seeto

Health Promotion Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

Original language	English (US)
Pages (from-to)	279-284
Number of pages	6
Journal	Alcohol
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/S0741-8329(98)00014-7
State	Published - Nov 1998

Keywords

Acetaldehyde
Alcohol Hair
ELISA Adducts

ASJC Scopus subject areas

Health(social science)
Biochemistry
Toxicology
Neurology
Behavioral Neuroscience

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10.1016/S0741-8329(98)00014-7

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title = "Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair",

abstract = "Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.",

keywords = "Acetaldehyde, Alcohol Hair, ELISA Adducts",

author = "Watson, {Ronald R.} and David Solkoff and Wang, {James Y.} and Keifong Seeto",

note = "Funding Information: This research was supported in part by NIH grant AA08037.",

year = "1998",

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doi = "10.1016/S0741-8329(98)00014-7",

language = "English (US)",

volume = "16",

pages = "279--284",

journal = "Alcohol",

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publisher = "Elsevier Inc.",

number = "4",

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TY - JOUR

T1 - Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

AU - Watson, Ronald R.

AU - Solkoff, David

AU - Wang, James Y.

AU - Seeto, Keifong

N1 - Funding Information: This research was supported in part by NIH grant AA08037.

PY - 1998/11

Y1 - 1998/11

N2 - Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

AB - Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

KW - Acetaldehyde

KW - Alcohol Hair

KW - ELISA Adducts

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DO - 10.1016/S0741-8329(98)00014-7

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AN - SCOPUS:0031727906

SN - 0741-8329

VL - 16

SP - 279

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JO - Alcohol

JF - Alcohol

IS - 4

ER -

Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

Abstract

Keywords

ASJC Scopus subject areas

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