Detection of avian influenza antigens in proximity fiber, droplet and optical waveguide microfluidics

Jeong Yeol Yoon, Brian C. Heinze, Jessica Gamboa, David J. You

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Virus antigens of avian influenza subtype H3N2 were detected on two different microfluidic platforms: microchannel and droplet. Latex immunoagglutination assays were performed using 920-nm highly carboxylated polystyrene beads that are conjugated with antibody to avian influenza virus. The bead suspension was merged with the solutions of avian influenza virus antigens in a Y-junction of a microchannel made by polydimethylsiloxane soft lithography. The resulting latex immunoagglutinations were measured with two optical fibers in proximity setup to detect 45° forward light scattering. Alternatively, 10 μL droplets of a bead suspension and an antigen solution were merged on a superhydrophobic surface (water contact angle = 155°), whose movement was guided by a metal wire, and 180° back light scattering is measured with a backscattering optical probe. Detection limits were 0.1 pg mL-1 for both microchannel with proximity fibers and droplet microfluidics, thanks to the use of micro-positioning stages to help generate reproducible optical signals. Additionally, optical waveguide was tested by constructing optical waveguide channels (filled with mineral oil) within a microfluidic device to detect the same light scattering. Detection limit was 0.1 ng mL-1 for an optical waveguide device, with a strong potential of improvement in the near future. The use of optical waveguide enabled smaller device setup, easier operation, smaller standard deviations and broader linear range of assay than proximity fiber microchannel and droplet microfluidics. Total assay time was less than 10 min.

Original languageEnglish (US)
Title of host publicationSmart Biomedical and Physiological Sensor Technology VI
StatePublished - 2009
EventSmart Biomedical and Physiological Sensor Technology VI - Orlando, FL, United States
Duration: Apr 16 2009Apr 17 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherSmart Biomedical and Physiological Sensor Technology VI
Country/TerritoryUnited States
CityOrlando, FL


  • Bird flu
  • Latex immunoagglutination assay
  • Light scattering
  • Optical fiber
  • Superhydrophobic surface

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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