TY - JOUR
T1 - Detecting serum antibodies to a purified recombinant proline-rich antigen of Coccidioides immitis in patients with coccidioidomycosis
AU - Orsborn, Kris I.
AU - Galgiani, John N.
N1 - Funding Information:
Received 27 March 1998; revised 5 August 1998. Financial support: This work was supported in part by the U.S. Department of Veterans Affairs. Reprints or correspondence: Dr. John N. Galgiani, Valley Fever Center for Excellence, 3601 South Sixth Avenue, Tucson, Arizona 85723.
PY - 1998/12
Y1 - 1998/12
N2 - In previous work, antibodies in serum samples from patients with coccidioidomycosis were found to react with a proline-rich antigen (PRA) isolated from spherules of Coccidioides immitis, and the gene encoding this antigen was cloned. We expressed and purified recombinant PRA (rPRA) by removing the majority of amino acids contributed by the vector from the fusion protein. Purified rPRA reacted with serum IgG antibodies in 37 of 42 patients with culture-proven progressive pulmonary or extrapulmonary coccidioidal disease; specific antibodies in dilutions ranging from 1:40 to 1:102,400 were demonstrated (sensitivity, 88%). In contrast, for >95% of patients without coccidioidomycosis reactivity of <1:40 was demonstrated (specificity, 97%). Of 18 patients with primary self-limited coccidioidomycosis, none had detectable antibodies in serum samples collected up to 141 days after illness began. The association of antibodies to rPRA with progressive infection may have prognostic value.
AB - In previous work, antibodies in serum samples from patients with coccidioidomycosis were found to react with a proline-rich antigen (PRA) isolated from spherules of Coccidioides immitis, and the gene encoding this antigen was cloned. We expressed and purified recombinant PRA (rPRA) by removing the majority of amino acids contributed by the vector from the fusion protein. Purified rPRA reacted with serum IgG antibodies in 37 of 42 patients with culture-proven progressive pulmonary or extrapulmonary coccidioidal disease; specific antibodies in dilutions ranging from 1:40 to 1:102,400 were demonstrated (sensitivity, 88%). In contrast, for >95% of patients without coccidioidomycosis reactivity of <1:40 was demonstrated (specificity, 97%). Of 18 patients with primary self-limited coccidioidomycosis, none had detectable antibodies in serum samples collected up to 141 days after illness began. The association of antibodies to rPRA with progressive infection may have prognostic value.
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U2 - 10.1086/515041
DO - 10.1086/515041
M3 - Article
C2 - 9868663
AN - SCOPUS:0032466510
SN - 1058-4838
VL - 27
SP - 1475
EP - 1478
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
IS - 6
ER -