Destruction of preantral follicles in adult rats by 4-vinyl-1-cyclohexene diepoxide

Jodi A. Flaws, Julie K. Doerr, I. Glenn Sipes, Patricia B. Hoyer

Research output: Contribution to journalArticlepeer-review

110 Scopus citations


4-vinyl-1-cyclohexene diepoxide (VCD) is known to destroy oocytes in ovaries of immature rats. Since ovaries functionally differ between immature and adult animals, we examined the effect of VCD on oocytes in adult rats. Adult (58 days) and immature (28 days) rats were injected daily (30 days) with vehicle or VCD. Each group contained 10 rats. During this time, cyclicity was determined daily by vaginal cytology. Animals were terminated on day 31 and tissues were collected. Oocytes were counted; livers, spleens, and uteri were weighed. VCD reduced the number of regular estrous cycles/30 days in adults, but not immature rats (n = 20, P < 0.05). VCD reduced the number of oocytes in adult and immature rats (n = 20, P < 0.05). Liver, spleen, or ovarian weights were not affected by VCD in either group. VCD reduced ruterine weight in adult (n = 20, P < 0.05) but not in immature rats. These results demonstrate that VCD decreases uterine weight in adult rats and as with immature rats, selectively destroys oocytes in ovaries of adults.

Original languageEnglish (US)
Pages (from-to)509-514
Number of pages6
JournalReproductive Toxicology
Issue number6
StatePublished - 1994


  • VCD
  • adult rats
  • follicle
  • infertility
  • ovarian toxicity
  • uterine toxicity

ASJC Scopus subject areas

  • Toxicology


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