Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows

Grant T. Shimamoto, John C. Byatt, Michael G. Jennings, Patricia G. Comens-Keller, Robert J. Collier

    Research output: Contribution to journalArticlepeer-review

    10 Scopus citations


    Total somatomedins from milk of bovine somatotropin-treated cows were isolated and characterized to determine the relative amount of the three amino acid N-terminally truncated form of IGF-I (destripeptide IGF-I). The somatomedin fraction was isolated using organic solvent and solid-phase extractions followed by preparative reverse phase HPLC and affinity chromatography. The overall yield of IGF-I was 28%, and destripeptide IGF-I was recovered with similar efficiency. The isolated somatomedins were resolved by capillary zonal electrophoresis and identified using recombinant somatomedin standards. The concentration of destripeptide IGF-I relative to full length IGF-I was determined by amino terminal sequencing and by bioassay. Results from these experiments indicated that the level of destripeptide IGF-I in milk from somatotropin-treated cows was less than 3% of the IGF-I concentration. Destripeptide IGF-I is therefore a minor component of the somatomedins present in milk from treated cows and does not contribute significantly to the proliferative activity of this milk.

    Original languageEnglish (US)
    Pages (from-to)296-300
    Number of pages5
    JournalPediatric Research
    Issue number3
    StatePublished - Sep 1992

    ASJC Scopus subject areas

    • Pediatrics, Perinatology, and Child Health


    Dive into the research topics of 'Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows'. Together they form a unique fingerprint.

    Cite this