Design of new strategy for green algal photo-hydrogen production: Spectral-selective photosystem I activation and photosystem II deactivation

Takanori Hoshino; Daniel J. Johnson; Joel L. Cuello

doi:10.1016/j.biortech.2012.06.011

Design of new strategy for green algal photo-hydrogen production: Spectral-selective photosystem I activation and photosystem II deactivation

Takanori Hoshino, Daniel J. Johnson, Joel L. Cuello

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A new strategy in photosynthetic hydrogen (photo-H₂) production from green algae was developed based on theory and successfully demonstrated. The new strategy applied a spectral-selective photosystem I (PSI) activating/photosystem II (PSII) deactivating radiation (or PSI light) that would drive a steady flow of electrons in the electron transport chain for delivery to hydrogenase for photo-H₂ production, but would reduce oxygen production through water photolysis below the respiratory oxygen consumption so that an anoxic condition would be maintained as required by hydrogenase. Implementing the strategy by using a PSI light (692nm peak, 680-700nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H₂ production (total of 0.108mLH₂mg^-1Chl, exceeding 0.066mLH₂mg^-1Chl under white light). The strategy also proved successful and convenient in allowing cells to alternately switch between photo-H₂ production and a recovery period by simply turning on or off the PSI light.

Original language	English (US)
Pages (from-to)	233-240
Number of pages	8
Journal	Bioresource Technology
Volume	120
DOIs	https://doi.org/10.1016/j.biortech.2012.06.011
State	Published - Sep 2012

Keywords

Algae
Chlamydomonas reinhardtii
Hydrogen
Light emitting diodes (LEDs)
Photosystem

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2012.06.011

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title = "Design of new strategy for green algal photo-hydrogen production: Spectral-selective photosystem I activation and photosystem II deactivation",

abstract = "A new strategy in photosynthetic hydrogen (photo-H2) production from green algae was developed based on theory and successfully demonstrated. The new strategy applied a spectral-selective photosystem I (PSI) activating/photosystem II (PSII) deactivating radiation (or PSI light) that would drive a steady flow of electrons in the electron transport chain for delivery to hydrogenase for photo-H2 production, but would reduce oxygen production through water photolysis below the respiratory oxygen consumption so that an anoxic condition would be maintained as required by hydrogenase. Implementing the strategy by using a PSI light (692nm peak, 680-700nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H2 production (total of 0.108mLH2mg-1Chl, exceeding 0.066mLH2mg-1Chl under white light). The strategy also proved successful and convenient in allowing cells to alternately switch between photo-H2 production and a recovery period by simply turning on or off the PSI light.",

keywords = "Algae, Chlamydomonas reinhardtii, Hydrogen, Light emitting diodes (LEDs), Photosystem",

author = "Takanori Hoshino and Johnson, {Daniel J.} and Cuello, {Joel L.}",

year = "2012",

month = sep,

doi = "10.1016/j.biortech.2012.06.011",

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AU - Johnson, Daniel J.

AU - Cuello, Joel L.

PY - 2012/9

Y1 - 2012/9

N2 - A new strategy in photosynthetic hydrogen (photo-H2) production from green algae was developed based on theory and successfully demonstrated. The new strategy applied a spectral-selective photosystem I (PSI) activating/photosystem II (PSII) deactivating radiation (or PSI light) that would drive a steady flow of electrons in the electron transport chain for delivery to hydrogenase for photo-H2 production, but would reduce oxygen production through water photolysis below the respiratory oxygen consumption so that an anoxic condition would be maintained as required by hydrogenase. Implementing the strategy by using a PSI light (692nm peak, 680-700nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H2 production (total of 0.108mLH2mg-1Chl, exceeding 0.066mLH2mg-1Chl under white light). The strategy also proved successful and convenient in allowing cells to alternately switch between photo-H2 production and a recovery period by simply turning on or off the PSI light.

AB - A new strategy in photosynthetic hydrogen (photo-H2) production from green algae was developed based on theory and successfully demonstrated. The new strategy applied a spectral-selective photosystem I (PSI) activating/photosystem II (PSII) deactivating radiation (or PSI light) that would drive a steady flow of electrons in the electron transport chain for delivery to hydrogenase for photo-H2 production, but would reduce oxygen production through water photolysis below the respiratory oxygen consumption so that an anoxic condition would be maintained as required by hydrogenase. Implementing the strategy by using a PSI light (692nm peak, 680-700nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H2 production (total of 0.108mLH2mg-1Chl, exceeding 0.066mLH2mg-1Chl under white light). The strategy also proved successful and convenient in allowing cells to alternately switch between photo-H2 production and a recovery period by simply turning on or off the PSI light.

KW - Algae

KW - Chlamydomonas reinhardtii

KW - Hydrogen

KW - Light emitting diodes (LEDs)

KW - Photosystem

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Design of new strategy for green algal photo-hydrogen production: Spectral-selective photosystem I activation and photosystem II deactivation

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Keywords

ASJC Scopus subject areas

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