Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy; Maged M. Henary; Nisan Siegel; Pa Dreyia V. Lawson; Yonggang Wu; Karin Schmidt; Jean Luc Brédas; Joseph W. Perry; Christoph J. Fahrni

doi:10.1021/ja073240o

Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy
, Maged M. Henary
, Nisan Siegel
, Pa Dreyia V. Lawson
, Yonggang Wu
, Karin Schmidt
, Jean Luc Brédas
, Joseph W. Perry
, Christoph J. Fahrni

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

Original language	English (US)
Pages (from-to)	11888-11889
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	39
DOIs	https://doi.org/10.1021/ja073240o
State	Published - Oct 3 2007
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja073240o

Cite this

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abstract = "To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.",

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doi = "10.1021/ja073240o",

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T1 - Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

AU - Sumalekshmy, S.

AU - Henary, Maged M.

AU - Siegel, Nisan

AU - Lawson, Pa Dreyia V.

AU - Wu, Yonggang

AU - Schmidt, Karin

AU - Brédas, Jean Luc

AU - Perry, Joseph W.

AU - Fahrni, Christoph J.

PY - 2007/10/3

Y1 - 2007/10/3

N2 - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

AB - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 39

ER -

Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

Abstract

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