Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy; Maged M. Henary; Nisan Siegel; Pa Dreyia V. Lawson; Yonggang Wu; Karin Schmidt; Jean Luc Brédas; Joseph W. Perry; Christoph J. Fahrni

doi:10.1021/ja073240o

Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy, Maged M. Henary, Nisan Siegel, Pa Dreyia V. Lawson, Yonggang Wu, Karin Schmidt, Jean Luc Brédas, Joseph W. Perry, Christoph J. Fahrni

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

Original language	English (US)
Pages (from-to)	11888-11889
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	39
DOIs	https://doi.org/10.1021/ja073240o
State	Published - Oct 3 2007
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.",

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T1 - Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

AU - Sumalekshmy, S.

AU - Henary, Maged M.

AU - Siegel, Nisan

AU - Lawson, Pa Dreyia V.

AU - Wu, Yonggang

AU - Schmidt, Karin

AU - Brédas, Jean Luc

AU - Perry, Joseph W.

AU - Fahrni, Christoph J.

PY - 2007/10/3

Y1 - 2007/10/3

N2 - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

AB - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

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Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

Abstract

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