Design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties

Natzem A. Lima, Travis W. Sawyer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Multispectral fluorescence imaging (MSFI) is a powerful imaging modality for tissue analysis and diagnostic imaging. By illuminating with distinct wavelengths of light, intrinsic biological fluorophores and labeled markers can be measured, providing information about tissue metabolism and function. MSFI has shown promise in the scope of gastrointestinal (GI) cancers such as colon and gastric cancers. Before MSFI can be used as an endoscopic diagnostic tool there requires extensive characterization of tissue properties to identify biomarker variations that occur with the onset of disease. A robust, whole organ imaging instrument to characterize autofluorescence properties would greatly inform the development of diagnostic imaging platforms. This paper reviews the design and validation of a multispectral fluorescence imaging system for characterizing whole organ tissue fluorescence and reflectance properties. We present a detailed discussion on design considerations and demonstrate excellent performance suitable to detect tissue autofluorescence.

Original languageEnglish (US)
Title of host publicationMultiscale Imaging and Spectroscopy III
EditorsPaul J. Campagnola, Kristen C. Maitland, Darren M. Roblyer
PublisherSPIE
ISBN (Electronic)9781510647596
DOIs
StatePublished - 2022
EventMultiscale Imaging and Spectroscopy III 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11944
ISSN (Print)1605-7422

Conference

ConferenceMultiscale Imaging and Spectroscopy III 2022
CityVirtual, Online
Period2/20/222/24/22

Keywords

  • Multispectral fluorescence imaging
  • gastrointestinal cancers
  • tissue fluorescence
  • whole-organ imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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